Rosmarinic acid attenuates TNF-α induced cardiomyocyte injury via regulating miR-344a-3p
To investigate whether rosmarinic acid protects cardiomyocytes from inflammatory damage through miRNAs, high-throughput sequencing and bioinformatics analysis were performed to identify TNF-α-induced inflammatory damage in cardiomyocytes and miRNAs differentially expressed in TNF-α-induced inflammatory injury in cardiomyocytes, and the bioinformatics analysis shown that the expression levels of 10 miRNAs were significantly up-regulated, and the expression levels of 6 miRNAs were significantly down-regulated. Among them, the expression level of miR-344a-3p was significantly up-regulated in the experimental group, while the expression level of miR-449c-5p was significantly down-regulated in experimental group of cells. The target genes of miR-344a-3p and miR-449c-5p were CCR1 and ATP2B4 respectively. The luciferase reporter system showed that luciferase activity in the WT-CCR1+miR-344a-3p mimic group was significantly decreased, and the expression of CCR1 was significantly decreased at mRNA and protein level after miR-344a-3p was transfected into H9C2 cells, indicating that TNF-α-induced inflammatory injury in cardiomyocytes, rosmarinic acid may up-regulate the expression of miR-344a-3p, thereby inhibiting the expression of CCR1 and ultimately protecting the cardiomyocytes from inflammatory damage. Thus, we thought that CCR1 might be a new therapeutic target for cardiomyocyte injury.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:70 |
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| Enthalten in: |
Cellular and molecular biology (Noisy-le-Grand, France) - 70(2024), 1 vom: 31. Jan., Seite 207-212 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Jun [VerfasserIn] |
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| Links: |
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| Themen: |
EC 1.13.12.- |
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| Anmerkungen: |
Date Completed 20.02.2024 Date Revised 17.03.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.14715/cmb/2024.70.1.28 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM368621553 |
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| 245 | 1 | 0 | |a Rosmarinic acid attenuates TNF-α induced cardiomyocyte injury via regulating miR-344a-3p |
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| 520 | |a To investigate whether rosmarinic acid protects cardiomyocytes from inflammatory damage through miRNAs, high-throughput sequencing and bioinformatics analysis were performed to identify TNF-α-induced inflammatory damage in cardiomyocytes and miRNAs differentially expressed in TNF-α-induced inflammatory injury in cardiomyocytes, and the bioinformatics analysis shown that the expression levels of 10 miRNAs were significantly up-regulated, and the expression levels of 6 miRNAs were significantly down-regulated. Among them, the expression level of miR-344a-3p was significantly up-regulated in the experimental group, while the expression level of miR-449c-5p was significantly down-regulated in experimental group of cells. The target genes of miR-344a-3p and miR-449c-5p were CCR1 and ATP2B4 respectively. The luciferase reporter system showed that luciferase activity in the WT-CCR1+miR-344a-3p mimic group was significantly decreased, and the expression of CCR1 was significantly decreased at mRNA and protein level after miR-344a-3p was transfected into H9C2 cells, indicating that TNF-α-induced inflammatory injury in cardiomyocytes, rosmarinic acid may up-regulate the expression of miR-344a-3p, thereby inhibiting the expression of CCR1 and ultimately protecting the cardiomyocytes from inflammatory damage. Thus, we thought that CCR1 might be a new therapeutic target for cardiomyocyte injury | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 7 | |a Rosmarinic Acid |2 NLM | |
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| 700 | 1 | |a Li, Yafei |e verfasserin |4 aut | |
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