Neural stem cell-derived exosomes regulate cell proliferation, migration, and cell death of brain microvascular endothelial cells via the miR-9/Hes1 axis under hypoxia
© 2024 The Authors. Animal Models and Experimental Medicine published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences..
BACKGROUND: Our previous study found that mouse embryonic neural stem cell (NSC)-derived exosomes (EXOs) regulated NSC differentiation via the miR-9/Hes1 axis. However, the effects of EXOs on brain microvascular endothelial cell (BMEC) dysfunction via the miR-9/Hes1 axis remain unknown. Therefore, the current study aimed to determine the effects of EXOs on BMEC proliferation, migration, and death via the miR-9/Hes1 axis.
METHODS: Immunofluorescence, quantitative real-time polymerase chain reaction, cell counting kit-8 assay, wound healing assay, calcein-acetoxymethyl/propidium iodide staining, and hematoxylin and eosin staining were used to determine the role and mechanism of EXOs on BMECs.
RESULTS: EXOs promoted BMEC proliferation and migration and reduced cell death under hypoxic conditions. The overexpression of miR-9 promoted BMEC proliferation and migration and reduced cell death under hypoxic conditions. Moreover, miR-9 downregulation inhibited BMEC proliferation and migration and also promoted cell death. Hes1 silencing ameliorated the effect of amtagomiR-9 on BMEC proliferation and migration and cell death. Hyperemic structures were observed in the regions of the hippocampus and cortex in hypoxia-induced mice. Meanwhile, EXO treatment improved cerebrovascular alterations.
CONCLUSION: NSC-derived EXOs can promote BMEC proliferation and migration and reduce cell death via the miR-9/Hes1 axis under hypoxic conditions. Therefore, EXO therapeutic strategies could be considered for hypoxia-induced vascular injury.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
|---|---|
| Enthalten in: |
Animal models and experimental medicine - 7(2024), 1 vom: 16. Feb., Seite 24-35 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Deng, Xiaojun [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Brain microvascular endothelial cells |
|---|
| Anmerkungen: |
Date Completed 26.03.2024 Date Revised 27.03.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1002/ame2.12394 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM368597458 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM368597458 | ||
| 003 | DE-627 | ||
| 005 | 20240327235835.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240219s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/ame2.12394 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1351.xml |
| 035 | |a (DE-627)NLM368597458 | ||
| 035 | |a (NLM)38369683 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Deng, Xiaojun |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Neural stem cell-derived exosomes regulate cell proliferation, migration, and cell death of brain microvascular endothelial cells via the miR-9/Hes1 axis under hypoxia |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 26.03.2024 | ||
| 500 | |a Date Revised 27.03.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2024 The Authors. Animal Models and Experimental Medicine published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences. | ||
| 520 | |a BACKGROUND: Our previous study found that mouse embryonic neural stem cell (NSC)-derived exosomes (EXOs) regulated NSC differentiation via the miR-9/Hes1 axis. However, the effects of EXOs on brain microvascular endothelial cell (BMEC) dysfunction via the miR-9/Hes1 axis remain unknown. Therefore, the current study aimed to determine the effects of EXOs on BMEC proliferation, migration, and death via the miR-9/Hes1 axis | ||
| 520 | |a METHODS: Immunofluorescence, quantitative real-time polymerase chain reaction, cell counting kit-8 assay, wound healing assay, calcein-acetoxymethyl/propidium iodide staining, and hematoxylin and eosin staining were used to determine the role and mechanism of EXOs on BMECs | ||
| 520 | |a RESULTS: EXOs promoted BMEC proliferation and migration and reduced cell death under hypoxic conditions. The overexpression of miR-9 promoted BMEC proliferation and migration and reduced cell death under hypoxic conditions. Moreover, miR-9 downregulation inhibited BMEC proliferation and migration and also promoted cell death. Hes1 silencing ameliorated the effect of amtagomiR-9 on BMEC proliferation and migration and cell death. Hyperemic structures were observed in the regions of the hippocampus and cortex in hypoxia-induced mice. Meanwhile, EXO treatment improved cerebrovascular alterations | ||
| 520 | |a CONCLUSION: NSC-derived EXOs can promote BMEC proliferation and migration and reduce cell death via the miR-9/Hes1 axis under hypoxic conditions. Therefore, EXO therapeutic strategies could be considered for hypoxia-induced vascular injury | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Hes1 | |
| 650 | 4 | |a brain microvascular endothelial cells | |
| 650 | 4 | |a exosomes | |
| 650 | 4 | |a miR‐9 | |
| 650 | 4 | |a neural stem cells | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 650 | 7 | |a Hes1 protein, mouse |2 NLM | |
| 650 | 7 | |a Transcription Factor HES-1 |2 NLM | |
| 650 | 7 | |a MIRN9 microRNA, mouse |2 NLM | |
| 700 | 1 | |a Hu, Xiaoyi |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Shang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Wei, Yaqin |e verfasserin |4 aut | |
| 700 | 1 | |a Fu, Jiaqi |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Wenhui |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jinming |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Caicai |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Lili |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Ping |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Animal models and experimental medicine |d 2018 |g 7(2024), 1 vom: 16. Feb., Seite 24-35 |w (DE-627)NLM285083201 |x 2576-2095 |7 nnns |
| 773 | 1 | 8 | |g volume:7 |g year:2024 |g number:1 |g day:16 |g month:02 |g pages:24-35 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/ame2.12394 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 7 |j 2024 |e 1 |b 16 |c 02 |h 24-35 | ||