An active derivatization detection method for inline monitoring the isolation of carbohydrates by preparative liquid chromatography
Copyright © 2024. Published by Elsevier B.V..
Polysaccharides have unique physio-chemical properties and various biological functions and have rapidly expanded interest over the last two decades. The purification of polysaccharides and their degraded oligosaccharides is challenging because carbohydrates have no chromophore and need a proper detector to monitor the chromatographic elution process. This study proposed an active derivatization detection (ADD) method based on active splitting from post-column flow, a microchannel reactor for efficient derivatization of polysaccharide reducing sugars with p-hydroxybenzoic acid hydrazide, and in-line detection by the UV detector of liquid chromatography system. The method and device were validated by the use of 11 monosaccharides, sulfated oligosaccharides (from degraded carrageenan), and polysaccharides (from Zizania latifolia). It has shown much better performance than the traditional phenol-sulfuric acid method (gold standard). Moreover, the ADD module presumes an add-in to the original preparative LC system, independent of the scale of the purification process and type of system. The developed method is versatile for chromatographic separation of carbohydrates and lays the foundation for their subsequent studies.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1719 |
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| Enthalten in: |
Journal of chromatography. A - 1719(2024) vom: 29. März, Seite 464730 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yao, Zhen [VerfasserIn] |
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| Links: |
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| Themen: |
Active derivatization detection |
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| Anmerkungen: |
Date Completed 11.03.2024 Date Revised 11.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.chroma.2024.464730 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM368574555 |
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| 500 | |a Date Revised 11.03.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024. Published by Elsevier B.V. | ||
| 520 | |a Polysaccharides have unique physio-chemical properties and various biological functions and have rapidly expanded interest over the last two decades. The purification of polysaccharides and their degraded oligosaccharides is challenging because carbohydrates have no chromophore and need a proper detector to monitor the chromatographic elution process. This study proposed an active derivatization detection (ADD) method based on active splitting from post-column flow, a microchannel reactor for efficient derivatization of polysaccharide reducing sugars with p-hydroxybenzoic acid hydrazide, and in-line detection by the UV detector of liquid chromatography system. The method and device were validated by the use of 11 monosaccharides, sulfated oligosaccharides (from degraded carrageenan), and polysaccharides (from Zizania latifolia). It has shown much better performance than the traditional phenol-sulfuric acid method (gold standard). Moreover, the ADD module presumes an add-in to the original preparative LC system, independent of the scale of the purification process and type of system. The developed method is versatile for chromatographic separation of carbohydrates and lays the foundation for their subsequent studies | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Active derivatization detection | |
| 650 | 4 | |a Polysaccharide | |
| 650 | 4 | |a Post-column derivatization | |
| 650 | 4 | |a Preparative liquid chromatography | |
| 650 | 4 | |a Purification | |
| 650 | 7 | |a Carbohydrates |2 NLM | |
| 650 | 7 | |a Oligosaccharides |2 NLM | |
| 650 | 7 | |a Polysaccharides |2 NLM | |
| 700 | 1 | |a Zhu, Kehan |e verfasserin |4 aut | |
| 700 | 1 | |a Gu, Tianyi |e verfasserin |4 aut | |
| 700 | 1 | |a Schmitz, Oliver J |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Duxin |e verfasserin |4 aut | |
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