Duck plague virus Us3 regulates the expression of pUL48
Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved..
Duck plague (DP) is one of the contagious diseases caused by Duck plague virus (DPV), which is a serious threat to the development of duck farming. Us3 is a PKA-like protein kinase in alphaherpesvirus, which can regulate the biological functions of many viral proteins, but whether Us3 regulates pUL48 protein has not been reported. In this paper, Western Blot, qRT-PCR, dual luciferase reporter system and Co-IP were used to investigate the relationship between pUL48 and Us3. The results showed that: 1) pUL48 interacted with Us3 at 138-256aa through its DBD region. 2) Us3 enhanced the protein expression of pUL48 in a dose-dependent manner. 3) Us3 promoted the mRNA level of pUL48 by activating its promoter activity. 4) Us3 inhibited the transcriptional activation function of pUL48. The results can provide scientific data for perfecting and supplementing the function of alpha herpesvirus Us3 and pUL48.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:103 |
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| Enthalten in: |
Poultry science - 103(2024), 4 vom: 22. März, Seite 103498 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhou, Tong [VerfasserIn] |
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| Links: |
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| Themen: |
Duck plague virus |
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| Anmerkungen: |
Date Completed 22.03.2024 Date Revised 22.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.psj.2024.103498 |
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| funding: |
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NLM368546829 |
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| 520 | |a Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Duck plague (DP) is one of the contagious diseases caused by Duck plague virus (DPV), which is a serious threat to the development of duck farming. Us3 is a PKA-like protein kinase in alphaherpesvirus, which can regulate the biological functions of many viral proteins, but whether Us3 regulates pUL48 protein has not been reported. In this paper, Western Blot, qRT-PCR, dual luciferase reporter system and Co-IP were used to investigate the relationship between pUL48 and Us3. The results showed that: 1) pUL48 interacted with Us3 at 138-256aa through its DBD region. 2) Us3 enhanced the protein expression of pUL48 in a dose-dependent manner. 3) Us3 promoted the mRNA level of pUL48 by activating its promoter activity. 4) Us3 inhibited the transcriptional activation function of pUL48. The results can provide scientific data for perfecting and supplementing the function of alpha herpesvirus Us3 and pUL48 | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a duck plague virus | |
| 650 | 4 | |a pUL48 | |
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| 700 | 1 | |a Ruan, Peilin |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Mingshu |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Anchun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Tian, Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Qiao |e verfasserin |4 aut | |
| 700 | 1 | |a Ou, Xumin |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Di |e verfasserin |4 aut | |
| 700 | 1 | |a He, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Zhen |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Shaqiu |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Juan |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Xin-Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Yanling |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Dekang |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Shun |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Mafeng |e verfasserin |4 aut | |
| 700 | 1 | |a Jia, Renyong |e verfasserin |4 aut | |
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