Single-cell analysis of isoform switching and transposable element expression during preimplantation embryonic development
Copyright: © 2024 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited..
Alternative splicing is an essential regulatory mechanism for development and pathogenesis. Through alternative splicing one gene can encode multiple isoforms and be translated into proteins with different functions. Therefore, this diversity is an important dimension to understand the molecular mechanism governing embryo development. Isoform expression in preimplantation embryos has been extensively investigated, leading to the discovery of new isoforms. However, the dynamics of isoform switching of different types of transcripts throughout the development remains unexplored. Here, using single-cell direct isoform sequencing in over 100 single blastomeres from the mouse oocyte to blastocyst stage, we quantified isoform expression and found that 3-prime partial transcripts lacking stop codons are highly accumulated in oocytes and zygotes. These transcripts are not transcription by-products and might play a role in maternal to zygote transition (MZT) process. Long-read sequencing also enabled us to determine the expression of transposable elements (TEs) at specific loci. In this way, we identified 3,894 TE loci that exhibited dynamic changes along the preimplantation development, likely regulating the expression of adjacent genes. Our work provides novel insights into the transcriptional regulation of early embryo development.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
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| Enthalten in: |
PLoS biology - 22(2024), 2 vom: 27. Feb., Seite e3002505 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Chaoyang [VerfasserIn] |
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| Anmerkungen: |
Date Completed 04.03.2024 Date Revised 04.03.2024 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.1371/journal.pbio.3002505 |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Alternative splicing is an essential regulatory mechanism for development and pathogenesis. Through alternative splicing one gene can encode multiple isoforms and be translated into proteins with different functions. Therefore, this diversity is an important dimension to understand the molecular mechanism governing embryo development. Isoform expression in preimplantation embryos has been extensively investigated, leading to the discovery of new isoforms. However, the dynamics of isoform switching of different types of transcripts throughout the development remains unexplored. Here, using single-cell direct isoform sequencing in over 100 single blastomeres from the mouse oocyte to blastocyst stage, we quantified isoform expression and found that 3-prime partial transcripts lacking stop codons are highly accumulated in oocytes and zygotes. These transcripts are not transcription by-products and might play a role in maternal to zygote transition (MZT) process. Long-read sequencing also enabled us to determine the expression of transposable elements (TEs) at specific loci. In this way, we identified 3,894 TE loci that exhibited dynamic changes along the preimplantation development, likely regulating the expression of adjacent genes. Our work provides novel insights into the transcriptional regulation of early embryo development | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a DNA Transposable Elements |2 NLM | |
| 650 | 7 | |a Protein Isoforms |2 NLM | |
| 700 | 1 | |a Shi, Zhuoxing |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Qingpei |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Rong |e verfasserin |4 aut | |
| 700 | 1 | |a Su, Dan |e verfasserin |4 aut | |
| 700 | 1 | |a Chang, Lei |e verfasserin |4 aut | |
| 700 | 1 | |a Xiao, Chuanle |e verfasserin |4 aut | |
| 700 | 1 | |a Fan, Xiaoying |e verfasserin |4 aut | |
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