Bioaugmented ensiling of sweet sorghum with Pichia anomala and cellulase and improved enzymatic hydrolysis of silage via ball milling
Copyright © 2024 Elsevier Ltd. All rights reserved..
Sweet sorghum, as a seasonal energy crop, is rich in cellulose and hemicellulose that can be converted into biofuels. This work aims at investigating the effects of synergistic regulation of Pichia anomala and cellulase on ensiling quality and microbial community of sweet sorghum silages as a storage and pretreatment method. Furthermore, the combined pretreatment effects of ensiling and ball milling on sweet sorghum were evaluated by microstructure change and enzymatic hydrolysis. Based on membership function analysis, the combination of P. anomala and cellulase (PA + CE) significantly improved the silage quality by preserving organic components and promoting fermentation characteristics. The bioaugmented ensiling with PA + CE restructured the bacterial community by facilitating Lactobacillus and inhibiting undesired microorganisms by killer activity of P. anomala. The combined bioaugmented ensiling pretreatment with ball milling significantly increased the enzymatic hydrolysis efficiency (EHE) to 71%, accompanied by the increased specific surface area and decreased pore size/crystallinity of sweet sorghum. Moreover, the EHE after combined pretreatment was increased by 1.37 times compared with raw material. Hence, the combined pretreatment was demonstrated as a novel strategy to effectively enhance enzymatic hydrolysis of sweet sorghum.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:354 |
|---|---|
| Enthalten in: |
Journal of environmental management - 354(2024) vom: 09. März, Seite 120327 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Ren, Haiwei [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Ball milling pretreatment |
|---|
| Anmerkungen: |
Date Completed 11.03.2024 Date Revised 11.03.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.jenvman.2024.120327 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM368497143 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM368497143 | ||
| 003 | DE-627 | ||
| 005 | 20240311232323.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240216s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.jenvman.2024.120327 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1323.xml |
| 035 | |a (DE-627)NLM368497143 | ||
| 035 | |a (NLM)38359627 | ||
| 035 | |a (PII)S0301-4797(24)00313-X | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Ren, Haiwei |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Bioaugmented ensiling of sweet sorghum with Pichia anomala and cellulase and improved enzymatic hydrolysis of silage via ball milling |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 11.03.2024 | ||
| 500 | |a Date Revised 11.03.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024 Elsevier Ltd. All rights reserved. | ||
| 520 | |a Sweet sorghum, as a seasonal energy crop, is rich in cellulose and hemicellulose that can be converted into biofuels. This work aims at investigating the effects of synergistic regulation of Pichia anomala and cellulase on ensiling quality and microbial community of sweet sorghum silages as a storage and pretreatment method. Furthermore, the combined pretreatment effects of ensiling and ball milling on sweet sorghum were evaluated by microstructure change and enzymatic hydrolysis. Based on membership function analysis, the combination of P. anomala and cellulase (PA + CE) significantly improved the silage quality by preserving organic components and promoting fermentation characteristics. The bioaugmented ensiling with PA + CE restructured the bacterial community by facilitating Lactobacillus and inhibiting undesired microorganisms by killer activity of P. anomala. The combined bioaugmented ensiling pretreatment with ball milling significantly increased the enzymatic hydrolysis efficiency (EHE) to 71%, accompanied by the increased specific surface area and decreased pore size/crystallinity of sweet sorghum. Moreover, the EHE after combined pretreatment was increased by 1.37 times compared with raw material. Hence, the combined pretreatment was demonstrated as a novel strategy to effectively enhance enzymatic hydrolysis of sweet sorghum | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Ball milling pretreatment | |
| 650 | 4 | |a Bioaugmented ensiling | |
| 650 | 4 | |a Cellulase | |
| 650 | 4 | |a Enzymatic saccharification | |
| 650 | 4 | |a Pichia anomala | |
| 650 | 4 | |a Sweet sorghum | |
| 650 | 7 | |a Cellulase |2 NLM | |
| 650 | 7 | |a EC 3.2.1.4 |2 NLM | |
| 700 | 1 | |a Li, Jinlian |e verfasserin |4 aut | |
| 700 | 1 | |a Lan, Yuanyuan |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Nana |e verfasserin |4 aut | |
| 700 | 1 | |a Tian, Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jinping |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Zhiping |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Lianhua |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Yongming |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Yi |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of environmental management |d 2001 |g 354(2024) vom: 09. März, Seite 120327 |w (DE-627)NLM112866786 |x 1095-8630 |7 nnns |
| 773 | 1 | 8 | |g volume:354 |g year:2024 |g day:09 |g month:03 |g pages:120327 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.jenvman.2024.120327 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 354 |j 2024 |b 09 |c 03 |h 120327 | ||