Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection
© 2024. The Author(s)..
Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids.
| Errataetall: |
ErratumIn: Nat Commun. 2024 Mar 5;15(1):1993. - PMID 38443368 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Nature communications - 15(2024), 1 vom: 14. Feb., Seite 1366 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jeon, Eunyoung [VerfasserIn] |
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Date Completed 16.02.2024 Date Revised 08.03.2024 published: Electronic ErratumIn: Nat Commun. 2024 Mar 5;15(1):1993. - PMID 38443368 Citation Status MEDLINE |
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| doi: |
10.1038/s41467-024-45467-w |
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NLM368456579 |
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| 520 | |a Efficient pathogen enrichment and nucleic acid isolation are critical for accurate and sensitive diagnosis of infectious diseases, especially those with low pathogen levels. Our study introduces a biporous silica nanofilms-embedded sample preparation chip for pathogen and nucleic acid enrichment/isolation. This chip features unique biporous nanostructures comprising large and small pore layers. Computational simulations confirm that these nanostructures enhance the surface area and promote the formation of nanovortex, resulting in improved capture efficiency. Notably, the chip demonstrates a 100-fold lower limit of detection compared to conventional methods used for nucleic acid detection. Clinical validations using patient samples corroborate the superior sensitivity of the chip when combined with the luminescence resonance energy transfer assay. The enhanced sample preparation efficiency of the chip, along with the facile and straightforward synthesis of the biporous nanostructures, offers a promising solution for polymer chain reaction-free detection of nucleic acids | ||
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| 700 | 1 | |a Kim, Suyeon |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Jieun |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Yeonuk |e verfasserin |4 aut | |
| 700 | 1 | |a Jang, Hyowon |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Minju |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Sung-Han |e verfasserin |4 aut | |
| 700 | 1 | |a Kang, Taejoon |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Sang Kyung |e verfasserin |4 aut | |
| 700 | 1 | |a Kwak, Rhokyun |e verfasserin |4 aut | |
| 700 | 1 | |a Shin, Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Joonseok |e verfasserin |4 aut | |
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