Mitochondria-Associated Protein FgNdk1 Regulates the Development, Pathogenicity, and SDHI Fungicide Sensitivity of Fusarium graminearum by Interacting with Succinate Dehydrogenase
Nucleoside diphosphate kinase (NDK) plays an important role in many cellular processes in all organisms. In this study, we functionally characterized a nucleoside diphosphate kinase (FgNdk1) in Fusarium graminearum, a causal agent of Fusarium head blight (FHB). FgNdk1 was involved in the generation of energy in the electron-transfer chain by interacting with succinate dehydrogenase (FgSdhA, FgSdhC1, and FgSdhC2). Deletion of FgNdk1 not only resulted in abnormal mitochondrial morphology, decreased ATP content, defective fungal development, and impairment in the formation of the toxisome but also led to the suppressed expression level of DON biosynthesis enzymes, decreased DON biosynthesis, and declined pathogenicity as well. Furthermore, deletion of FgNdk1 caused increasing transcriptional levels of FgSdhC1 and FgdhC2, in the presence of pydiflumetofen, related to the decreased sensitivity to SDHI fungicides. Overall, this study identified a new regulatory mechanism of FgNdk1 in the pathogenicity and SDHI fungicide sensitivity of Fusarium graminearum.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:72 |
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| Enthalten in: |
Journal of agricultural and food chemistry - 72(2024), 8 vom: 28. Feb., Seite 3913-3925 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Chenguang [VerfasserIn] |
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| Links: |
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| Themen: |
EC 1.3.99.1 |
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| Anmerkungen: |
Date Completed 29.02.2024 Date Revised 29.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/acs.jafc.3c07934 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM368454029 |
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| 245 | 1 | 0 | |a Mitochondria-Associated Protein FgNdk1 Regulates the Development, Pathogenicity, and SDHI Fungicide Sensitivity of Fusarium graminearum by Interacting with Succinate Dehydrogenase |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Nucleoside diphosphate kinase (NDK) plays an important role in many cellular processes in all organisms. In this study, we functionally characterized a nucleoside diphosphate kinase (FgNdk1) in Fusarium graminearum, a causal agent of Fusarium head blight (FHB). FgNdk1 was involved in the generation of energy in the electron-transfer chain by interacting with succinate dehydrogenase (FgSdhA, FgSdhC1, and FgSdhC2). Deletion of FgNdk1 not only resulted in abnormal mitochondrial morphology, decreased ATP content, defective fungal development, and impairment in the formation of the toxisome but also led to the suppressed expression level of DON biosynthesis enzymes, decreased DON biosynthesis, and declined pathogenicity as well. Furthermore, deletion of FgNdk1 caused increasing transcriptional levels of FgSdhC1 and FgdhC2, in the presence of pydiflumetofen, related to the decreased sensitivity to SDHI fungicides. Overall, this study identified a new regulatory mechanism of FgNdk1 in the pathogenicity and SDHI fungicide sensitivity of Fusarium graminearum | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a FgNdk1 | |
| 650 | 4 | |a Fusarium graminearum | |
| 650 | 4 | |a NDK domain | |
| 650 | 4 | |a SDHI fungicides sensitivity | |
| 650 | 4 | |a pathogenicity | |
| 650 | 7 | |a Succinate Dehydrogenase |2 NLM | |
| 650 | 7 | |a EC 1.3.99.1 |2 NLM | |
| 650 | 7 | |a Fungicides, Industrial |2 NLM | |
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| 700 | 1 | |a Zhao, Feifei |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, ZhiWen |e verfasserin |4 aut | |
| 700 | 1 | |a Cai, Xiaowei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Mingguo |e verfasserin |4 aut | |
| 700 | 1 | |a Hou, Yiping |e verfasserin |4 aut | |
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