Production of acetic acid from wheat bran by catalysis of an acetoxylan esterase
Copyright © 2024 Elsevier Ltd. All rights reserved..
In this study, a gene encoding for acetylxylan esterase was cloned and expressed in E. coli. A single uniform band with molecular weight of 31.2 kDa was observed in SDS-PAGE electrophoresis. Served as the substrate, p-nitrophenol butyrate was employed to detect the recombinant enzyme activity. It exhibited activity at a wide temperature range (30-100 °C) and pH (5.0-9.0) with the optimal temperature of 70 °C and pH 8.0. Acetylxylan esterase showed two substrates' specificities with the highest Vmax of 177.2 U/mg and Km of 20.98 mM against p-nitrophenol butyrate. Meanwhile, the Vmax of p-nitrophenol acetate was 137.0 U/mg and Km 12.16 mM. The acetic acid yield of 0.39 g/g was obtained (70 °C and pH 8.0) from wheat bran pretreated using amylase and papain. This study showed the highest yield up to date and developed a promising strategy for acetic acid production using wheat bran.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:396 |
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| Enthalten in: |
Bioresource technology - 396(2024) vom: 15. März, Seite 130443 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Mei [VerfasserIn] |
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| Links: |
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| Themen: |
4-nitrophenol |
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| Anmerkungen: |
Date Completed 01.03.2024 Date Revised 01.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.biortech.2024.130443 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a In this study, a gene encoding for acetylxylan esterase was cloned and expressed in E. coli. A single uniform band with molecular weight of 31.2 kDa was observed in SDS-PAGE electrophoresis. Served as the substrate, p-nitrophenol butyrate was employed to detect the recombinant enzyme activity. It exhibited activity at a wide temperature range (30-100 °C) and pH (5.0-9.0) with the optimal temperature of 70 °C and pH 8.0. Acetylxylan esterase showed two substrates' specificities with the highest Vmax of 177.2 U/mg and Km of 20.98 mM against p-nitrophenol butyrate. Meanwhile, the Vmax of p-nitrophenol acetate was 137.0 U/mg and Km 12.16 mM. The acetic acid yield of 0.39 g/g was obtained (70 °C and pH 8.0) from wheat bran pretreated using amylase and papain. This study showed the highest yield up to date and developed a promising strategy for acetic acid production using wheat bran | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Acetic acid | |
| 650 | 4 | |a Acetylxylan esterase | |
| 650 | 4 | |a Enzyme pretreatment | |
| 650 | 4 | |a Wheat bran | |
| 650 | 7 | |a Esterases |2 NLM | |
| 650 | 7 | |a EC 3.1.- |2 NLM | |
| 650 | 7 | |a 4-nitrophenol |2 NLM | |
| 650 | 7 | |a Y92ZL45L4R |2 NLM | |
| 650 | 7 | |a Dietary Fiber |2 NLM | |
| 650 | 7 | |a Acetic Acid |2 NLM | |
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| 650 | 7 | |a Butyrates |2 NLM | |
| 650 | 7 | |a Nitrophenols |2 NLM | |
| 700 | 1 | |a Tang, Hongzhi |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Haiyang |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiaoji |e verfasserin |4 aut | |
| 700 | 1 | |a Xue, Dongsheng |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Xun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jiaqi |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Jia |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Chongju |e verfasserin |4 aut | |
| 700 | 1 | |a Gong, Chunjie |e verfasserin |4 aut | |
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