RNA-binding protein IGF2BP2 suppresses metastasis of clear cell renal cell carcinoma by enhancing CKB mRNA stability and expression
Copyright © 2024. Published by Elsevier Inc..
Clear cell renal cell carcinoma (ccRCC) is the most prevalent kidney cancer, with a highly aggressive phenotype and poor prognosis. RNA binding proteins (RBPs) play crucial roles in post-transcriptional gene regulation and have been implicated in tumorigenesis. RBPs have the potential to become a new therapeutic target for ccRCC. In this study, we screened and validated that insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) as an RBP, was down-regulated in ccRCC tissues and cell lines. Functionally, we verified that IGF2BP2 significantly suppressed the migration and invasion ability of ccRCC in vitro and in vivo. Mechanistically, RIP-seq and actinomycin D experiments results showed that IGF2BP2 enhanced the expression of Creatine Kinase B (CKB) by binding to CKB mRNA and enhancing its mRNA stability. Thus, IGF2BP2 inhibited ccRCC metastasis through enhancing the expression of CKB. Taken together, these finding suggests that IGF2BP2 is a novel metastasis suppressor of ccRCC and may serve as a potential therapeutic target.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:42 |
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| Enthalten in: |
Translational oncology - 42(2024) vom: 08. Feb., Seite 101904 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ren, Junwu [VerfasserIn] |
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| Links: |
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| Themen: |
CKB |
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| Anmerkungen: |
Date Revised 23.02.2024 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.tranon.2024.101904 |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Clear cell renal cell carcinoma (ccRCC) is the most prevalent kidney cancer, with a highly aggressive phenotype and poor prognosis. RNA binding proteins (RBPs) play crucial roles in post-transcriptional gene regulation and have been implicated in tumorigenesis. RBPs have the potential to become a new therapeutic target for ccRCC. In this study, we screened and validated that insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) as an RBP, was down-regulated in ccRCC tissues and cell lines. Functionally, we verified that IGF2BP2 significantly suppressed the migration and invasion ability of ccRCC in vitro and in vivo. Mechanistically, RIP-seq and actinomycin D experiments results showed that IGF2BP2 enhanced the expression of Creatine Kinase B (CKB) by binding to CKB mRNA and enhancing its mRNA stability. Thus, IGF2BP2 inhibited ccRCC metastasis through enhancing the expression of CKB. Taken together, these finding suggests that IGF2BP2 is a novel metastasis suppressor of ccRCC and may serve as a potential therapeutic target | ||
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| 650 | 4 | |a Clear cell renal cell carcinoma | |
| 650 | 4 | |a IGF2BP2 | |
| 650 | 4 | |a RNA binding protein | |
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| 700 | 1 | |a Li, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yongquan |e verfasserin |4 aut | |
| 700 | 1 | |a Pan, Xiaojuan |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Qiang |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yuying |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiaolin |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Ce |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yuying |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Shimin |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Feifei |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Haiping |e verfasserin |4 aut | |
| 700 | 1 | |a Ning, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Qin, Changhong |e verfasserin |4 aut | |
| 700 | 1 | |a Ran, Ai |e verfasserin |4 aut | |
| 700 | 1 | |a Xiao, Bin |e verfasserin |4 aut | |
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