A rapid immunomagnetic beads-based sELISA method for the detection of bovine αs1-casein based on specific epitopes
Copyright © 2024 Elsevier Ltd. All rights reserved..
Although αs1-casein poses significant health risks to individuals with milk allergies, the availability of quantification methods for this allergen remains limited. In this study, we developed an immunomagnetic beads-based immunoassay (IMBs-ELISA) for the precise quantitative detection of bovine αs1-CN, specifically targeting epitope AA173-194. No cross-reactivity was observed with the other 7 food allergens including milk allergen. The linear detection range of the established IMBs-ELISA method was 0.125 μg/mL-2.000 μg/mL, with a limit of detection of 0.099 μg/mL. The accuracy of this method was 1.048 %, and the intra-plate and inter-plate precision achieved 4.100 % and 6.777 %, respectively. Notably, the entire IMBs-ELISA process could be completed within 75 min, representing a substantial time-saving advantage over traditional ELISA methods. These results proved the reliability and rapidity of the IMBs-ELISA method for detecting αs1-CN in real food.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:444 |
|---|---|
| Enthalten in: |
Food chemistry - 444(2024) vom: 30. März, Seite 138565 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Tan, Hongkai [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Allergens |
|---|
| Anmerkungen: |
Date Completed 11.03.2024 Date Revised 11.03.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.foodchem.2024.138565 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM368300196 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM368300196 | ||
| 003 | DE-627 | ||
| 005 | 20240311232254.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240211s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.foodchem.2024.138565 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1323.xml |
| 035 | |a (DE-627)NLM368300196 | ||
| 035 | |a (NLM)38340505 | ||
| 035 | |a (PII)S0308-8146(24)00213-9 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Tan, Hongkai |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A rapid immunomagnetic beads-based sELISA method for the detection of bovine αs1-casein based on specific epitopes |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 11.03.2024 | ||
| 500 | |a Date Revised 11.03.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024 Elsevier Ltd. All rights reserved. | ||
| 520 | |a Although αs1-casein poses significant health risks to individuals with milk allergies, the availability of quantification methods for this allergen remains limited. In this study, we developed an immunomagnetic beads-based immunoassay (IMBs-ELISA) for the precise quantitative detection of bovine αs1-CN, specifically targeting epitope AA173-194. No cross-reactivity was observed with the other 7 food allergens including milk allergen. The linear detection range of the established IMBs-ELISA method was 0.125 μg/mL-2.000 μg/mL, with a limit of detection of 0.099 μg/mL. The accuracy of this method was 1.048 %, and the intra-plate and inter-plate precision achieved 4.100 % and 6.777 %, respectively. Notably, the entire IMBs-ELISA process could be completed within 75 min, representing a substantial time-saving advantage over traditional ELISA methods. These results proved the reliability and rapidity of the IMBs-ELISA method for detecting αs1-CN in real food | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Alpha s1-casein | |
| 650 | 4 | |a Epitope-based antibody | |
| 650 | 4 | |a IMBs-ELISA | |
| 650 | 4 | |a Milk allergen | |
| 650 | 4 | |a Quantification | |
| 650 | 7 | |a Epitopes |2 NLM | |
| 650 | 7 | |a Caseins |2 NLM | |
| 650 | 7 | |a Allergens |2 NLM | |
| 700 | 1 | |a Qiu, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Siyi |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Xintong |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Yong |e verfasserin |4 aut | |
| 700 | 1 | |a He, Shengfa |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Hongbing |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Food chemistry |d 1995 |g 444(2024) vom: 30. März, Seite 138565 |w (DE-627)NLM076785823 |x 1873-7072 |7 nnns |
| 773 | 1 | 8 | |g volume:444 |g year:2024 |g day:30 |g month:03 |g pages:138565 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.foodchem.2024.138565 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 444 |j 2024 |b 30 |c 03 |h 138565 | ||