Identification of rBlo t 41 with a chitin-binding type-2 domain : A novel major allergen from Blomia tropicalis
Copyright © 2024 Elsevier B.V. All rights reserved..
BACKGROUND: Blomia tropicalis (B. tropicalis) has been reported to impose an increased risk of allergic diseases. However, few characteristics of the unknown allergen components responsible for B. tropicalis allergy and clinical relevance have been fully identified.
METHODS: We synthesized and characterized the physicochemical properties and cross-reactivity of the newly discovered recombinant B. tropicalis group 41 allergen (rBlo t 41). Subsequently, sera were collected from 107 B. tropicalis allergic subjects to evaluate the prevalence of the rBlo t 41. Lastly, its allergenicity was tested in humans by basophil activation assays, and in mice by a model of allergic asthma.
RESULTS: The mature protein of rBlo t 41 was described as 104 amino acids long and 15.8 kDa, and its limited cross-reactivity was observed between allergens of house dust mites (HDM). Sensitization rate of rBlo t 41 (56.07 %) was lower than rBlo t 2 (76.29 %) and rBlo t 5 (69.07 %) in our study. Besides, rBlo t 41 elicited CD63 upregulation in basophils, whereas rBlo t 41-sensitized mice generated rBlo t 41-IgE and developed allergic airway inflammation after allergen exposure. Of note, component-based tests showed a high area under curve value (AUC = 0.75) of rBlo t 41, displaying its favorable diagnostic potential in B. tropicalis allergy.
CONCLUSIONS: rBlo t 41 was identified as a candidate novel major allergen with good diagnostic potential in B. tropicalis sensitization. Additionally, we provided strong evidence about rBlo t 41 on the clinically relevant manifestations in B. tropicalis allergies, conducive to facilitating the development of component-resolved diagnosis.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2024 |
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Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:262 |
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Enthalten in: |
International journal of biological macromolecules - 262(2024), Pt 1 vom: 15. März, Seite 129972 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Luo, Wenting [VerfasserIn] |
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Links: |
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Themen: |
1398-61-4 |
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Anmerkungen: |
Date Completed 20.03.2024 Date Revised 20.03.2024 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.ijbiomac.2024.129972 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM368258033 |
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500 | |a Citation Status MEDLINE | ||
520 | |a Copyright © 2024 Elsevier B.V. All rights reserved. | ||
520 | |a BACKGROUND: Blomia tropicalis (B. tropicalis) has been reported to impose an increased risk of allergic diseases. However, few characteristics of the unknown allergen components responsible for B. tropicalis allergy and clinical relevance have been fully identified | ||
520 | |a METHODS: We synthesized and characterized the physicochemical properties and cross-reactivity of the newly discovered recombinant B. tropicalis group 41 allergen (rBlo t 41). Subsequently, sera were collected from 107 B. tropicalis allergic subjects to evaluate the prevalence of the rBlo t 41. Lastly, its allergenicity was tested in humans by basophil activation assays, and in mice by a model of allergic asthma | ||
520 | |a RESULTS: The mature protein of rBlo t 41 was described as 104 amino acids long and 15.8 kDa, and its limited cross-reactivity was observed between allergens of house dust mites (HDM). Sensitization rate of rBlo t 41 (56.07 %) was lower than rBlo t 2 (76.29 %) and rBlo t 5 (69.07 %) in our study. Besides, rBlo t 41 elicited CD63 upregulation in basophils, whereas rBlo t 41-sensitized mice generated rBlo t 41-IgE and developed allergic airway inflammation after allergen exposure. Of note, component-based tests showed a high area under curve value (AUC = 0.75) of rBlo t 41, displaying its favorable diagnostic potential in B. tropicalis allergy | ||
520 | |a CONCLUSIONS: rBlo t 41 was identified as a candidate novel major allergen with good diagnostic potential in B. tropicalis sensitization. Additionally, we provided strong evidence about rBlo t 41 on the clinically relevant manifestations in B. tropicalis allergies, conducive to facilitating the development of component-resolved diagnosis | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Allergenicity | |
650 | 4 | |a Blomia tropicalis | |
650 | 4 | |a Cross-reactivity | |
650 | 4 | |a Storage mite | |
650 | 4 | |a rBlo t 41 | |
650 | 7 | |a Allergens |2 NLM | |
650 | 7 | |a Chitin |2 NLM | |
650 | 7 | |a 1398-61-4 |2 NLM | |
700 | 1 | |a Zhang, Jiale |e verfasserin |4 aut | |
700 | 1 | |a Zheng, Xianhui |e verfasserin |4 aut | |
700 | 1 | |a Li, Aoli |e verfasserin |4 aut | |
700 | 1 | |a Xv, Miaoyuan |e verfasserin |4 aut | |
700 | 1 | |a Zhou, Dongmei |e verfasserin |4 aut | |
700 | 1 | |a Yuan, Cunyin |e verfasserin |4 aut | |
700 | 1 | |a Cui, Yubao |e verfasserin |4 aut | |
700 | 1 | |a Sun, Baoqing |e verfasserin |4 aut | |
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