Cucumber (Cucumis sativus L.) with heterologous poly-γ-glutamic acid has skin moisturizing, whitening and anti-wrinkle effects
Copyright © 2024 Elsevier B.V. All rights reserved..
Three genes involved in poly-γ-glutamic acid(γ-PGA)synthesis cloned from Bacillus licheniformis were transformed into cucumber for the first time. Compared with control, its water content increased by 6-14 % and water loss rate decreased by 11-12 %. In zebrafish and human skin experiments, the moisturizing effect of transgenic cucumber was significantly higher than that of CK, γ-PGA and hyaluronic acid group. Transgenic cucumber reduced facial wrinkles and roughness by 19.58 % and 24.97 %, reduced skin melanin content by 5.27 %, increased skin topological angle and L-value by 5.89 % and 2.49 %, and increased the R2 and Q1 values of facial elasticity by 7.67 % and 5.64 %, respectively. The expressions of aqp3, Tyr, silv and OCA2 were down-regulated, eln1, eln2, col1a1a and col1a1b were up-regulated in zebrafish after treated with transgenic cucumber. This study provides an important reference for the endogenous synthesis of important skin care functional molecules in plants.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:262 |
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| Enthalten in: |
International journal of biological macromolecules - 262(2024), Pt 1 vom: 15. März, Seite 130026 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Can [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 20.03.2024 Date Revised 20.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ijbiomac.2024.130026 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Three genes involved in poly-γ-glutamic acid(γ-PGA)synthesis cloned from Bacillus licheniformis were transformed into cucumber for the first time. Compared with control, its water content increased by 6-14 % and water loss rate decreased by 11-12 %. In zebrafish and human skin experiments, the moisturizing effect of transgenic cucumber was significantly higher than that of CK, γ-PGA and hyaluronic acid group. Transgenic cucumber reduced facial wrinkles and roughness by 19.58 % and 24.97 %, reduced skin melanin content by 5.27 %, increased skin topological angle and L-value by 5.89 % and 2.49 %, and increased the R2 and Q1 values of facial elasticity by 7.67 % and 5.64 %, respectively. The expressions of aqp3, Tyr, silv and OCA2 were down-regulated, eln1, eln2, col1a1a and col1a1b were up-regulated in zebrafish after treated with transgenic cucumber. This study provides an important reference for the endogenous synthesis of important skin care functional molecules in plants | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Cucumber | |
| 650 | 4 | |a Heterologous synthesis | |
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| 700 | 1 | |a Ma, Haizhen |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Panpan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Shengkui |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Jieting |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Sheng, Wenlong |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Tianyu |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Lu |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Wanxia |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Tao |e verfasserin |4 aut | |
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