Ano1 regulates embryo transport by modulating intracellular calcium levels in oviduct smooth muscle
Copyright © 2024. Published by Elsevier B.V..
Oviductal smooth muscle exhibits spontaneous rhythmic contraction (SRC) and controls the passage of the ova at the exact time, but its mechanistic regulation remains to be determined. In this study, female mice with Ano1SMKO (smooth muscle-specific deletion of Ano1) had reduced fertility. Deficiency of Ano1 in mice resulted in impaired oviductal SRC function and reduced calcium signaling in individual smooth muscle cells in the oviduct. The Ano1 antagonist T16Ainh-A01 dose-dependently inhibited SRCs and [Ca2+]i in the oviducts of humans and mice. A similar inhibitory effect of SRCs and [Ca2+]i was observed after treatment with nifedipine. In our study, ANO1 acted primarily as an activator or amplifier in [Ca2+]i and contraction of tubal smooth muscle cells. We found that tubal SRC was markedly attenuated in patients with ectopic pregnancy. Then, our study was designed to determine whether chloride channel Ano1-mediated smooth muscle motility is associated with tubal SRC. Our findings reveal a new mechanism for the regulation of tubal motility that may be associated with abnormal pregnancies such as ectopic pregnancies.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1870 |
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| Enthalten in: |
Biochimica et biophysica acta. Molecular basis of disease - 1870(2024), 4 vom: 12. Apr., Seite 167059 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Xiao-Man [VerfasserIn] |
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| Links: |
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| Themen: |
[Ca(2+)](i) |
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| Anmerkungen: |
Date Completed 08.04.2024 Date Revised 15.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bbadis.2024.167059 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 520 | |a Oviductal smooth muscle exhibits spontaneous rhythmic contraction (SRC) and controls the passage of the ova at the exact time, but its mechanistic regulation remains to be determined. In this study, female mice with Ano1SMKO (smooth muscle-specific deletion of Ano1) had reduced fertility. Deficiency of Ano1 in mice resulted in impaired oviductal SRC function and reduced calcium signaling in individual smooth muscle cells in the oviduct. The Ano1 antagonist T16Ainh-A01 dose-dependently inhibited SRCs and [Ca2+]i in the oviducts of humans and mice. A similar inhibitory effect of SRCs and [Ca2+]i was observed after treatment with nifedipine. In our study, ANO1 acted primarily as an activator or amplifier in [Ca2+]i and contraction of tubal smooth muscle cells. We found that tubal SRC was markedly attenuated in patients with ectopic pregnancy. Then, our study was designed to determine whether chloride channel Ano1-mediated smooth muscle motility is associated with tubal SRC. Our findings reveal a new mechanism for the regulation of tubal motility that may be associated with abnormal pregnancies such as ectopic pregnancies | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Ano1 | |
| 650 | 4 | |a Ectopic pregnancy | |
| 650 | 4 | |a Fallopian/oviduct | |
| 650 | 4 | |a Smooth muscle | |
| 650 | 4 | |a Spontaneous rhythmic contraction | |
| 650 | 4 | |a [Ca(2+)](i) | |
| 650 | 7 | |a Calcium |2 NLM | |
| 650 | 7 | |a SY7Q814VUP |2 NLM | |
| 650 | 7 | |a Chloride Channels |2 NLM | |
| 650 | 7 | |a ANO1 protein, human |2 NLM | |
| 650 | 7 | |a ANO1 protein, mouse |2 NLM | |
| 700 | 1 | |a Li, Juan |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Defang |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Qin, Xiao-Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yun-Xia |e verfasserin |4 aut | |
| 700 | 1 | |a Gu, Yong-Zhong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Na |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Li-Guang |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Mei |e verfasserin |4 aut | |
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