A colorimetric detection of dopamine in urine and serum based on the CeO2 @ZIF-8/Cu-CDs laccase-like nanozyme activity
© 2024 John Wiley & Sons Ltd..
This study reports a sensitive and selective colorimetric approach for the analysis of dopamine (DA) based on CeO2 ZIF-8/Cu-CDs laccase-like nanozymes activity. The CeO2 @ZIF-8/Cu-CDs was synthesized using cerium oxide (CeO2 ) and copper-doped carbon dots (Cu-CDs) with 2-methylimidazole by a facilely hydrothermal approach. The CeO2 @ZIF-8/Cu-CDs exhibited excellent laccase-like nanozymes activity and can oxidize the colorless substrate (DA) to red product with 4-aminoantipyrine as the chromogenic agent. The Michaelis-Menten constant (Km ) and the maximal velocity (Vmax ) of CeO2 @ZIF-8/Cu-CDs are 0.20 mM and 1.48 μM/min, respectively. The detection method has a linear range of 0.05-7.5 μg/mL and a detection limit as low as 8.5 ng/mL with good reproducibility. The developed colorimetric sensor was applied to rapid and precise quantitative evaluation of DA levels in serum and urine samples. This study presents a new approach for detecting biological molecules by utilizing the controlled regulation of nanozymes' laccase-like activity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:39 |
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| Enthalten in: |
Luminescence : the journal of biological and chemical luminescence - 39(2024), 2 vom: 08. Feb., Seite e4684 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yin, Qinhong [VerfasserIn] |
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| Links: |
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| Themen: |
7440-44-0 |
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| Anmerkungen: |
Date Completed 14.02.2024 Date Revised 25.02.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1002/bio.4684 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM368219569 |
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| 520 | |a This study reports a sensitive and selective colorimetric approach for the analysis of dopamine (DA) based on CeO2 ZIF-8/Cu-CDs laccase-like nanozymes activity. The CeO2 @ZIF-8/Cu-CDs was synthesized using cerium oxide (CeO2 ) and copper-doped carbon dots (Cu-CDs) with 2-methylimidazole by a facilely hydrothermal approach. The CeO2 @ZIF-8/Cu-CDs exhibited excellent laccase-like nanozymes activity and can oxidize the colorless substrate (DA) to red product with 4-aminoantipyrine as the chromogenic agent. The Michaelis-Menten constant (Km ) and the maximal velocity (Vmax ) of CeO2 @ZIF-8/Cu-CDs are 0.20 mM and 1.48 μM/min, respectively. The detection method has a linear range of 0.05-7.5 μg/mL and a detection limit as low as 8.5 ng/mL with good reproducibility. The developed colorimetric sensor was applied to rapid and precise quantitative evaluation of DA levels in serum and urine samples. This study presents a new approach for detecting biological molecules by utilizing the controlled regulation of nanozymes' laccase-like activity | ||
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| 700 | 1 | |a Zhu, Yanqin |e verfasserin |4 aut | |
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