Bacterial heat shock protein : A new crosstalk between T lymphocyte and macrophage via JAK2/STAT1 pathway in bloodstream infection
Copyright © 2024. Published by Elsevier GmbH..
Bloodstream infection (BSI) refers to the infection of blood by pathogens. Severe immune response to BSI can lead to sepsis, a systemic infection leading to multiple organ dysfunction, coupled with drug resistance, mortality, and limited clinical treatment options. This work aims to further investigate the new interplay between bacterial exocrine regulatory protein and host immune cells in the context of highly drug-resistant malignant BSI. Whether interfering with related regulatory signaling pathways can reverse the inflammatory disorder of immune cells. In-depth analysis of single-cell sequencing results in Septic patients for potential immunodeficiency factors. Analysis of key proteins enriched by host cells and key pathways using proteomics. Cell models and animal models validate the pathological effects of DnaK on T cells, MAITs, macrophages, and osteoclasts. The blood of patients was analyzed for the immunosuppression of T cells and MAITs. We identified that S. maltophilia-DnaK was enriched in immunodeficient T cells. The activation of the JAK2/STAT1 axis initiated the exhaustion of T cells. Septic patients with Gram-negative bacterial infections exhibited deficiencies in MAITs, which correspond to IFN-γ. Cellular and animal experiments confirmed that DnaK could facilitate MAIT depletion and M1 polarization of macrophages. Additionally, Fludarabine mitigated M1 polarization of blood, liver, and spleen in mice. Interestingly, DnaK also repressed osteoclastogenesis of macrophages stimulated by RANKL. S.maltophilia-DnaK prompts the activation of the JAK2/STAT1 axis in T cells and the M1 polarization of macrophages. Targeting the DnaK's crosstalk can be a potentially effective approach for treating the inflammatory disorder in the broad-spectrum drug-resistant BSI.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:282 |
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| Enthalten in: |
Microbiological research - 282(2024) vom: 15. März, Seite 127626 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yin, Sheng [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 18.03.2024 Date Revised 18.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.micres.2024.127626 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 245 | 1 | 0 | |a Bacterial heat shock protein |b A new crosstalk between T lymphocyte and macrophage via JAK2/STAT1 pathway in bloodstream infection |
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| 520 | |a Copyright © 2024. Published by Elsevier GmbH. | ||
| 520 | |a Bloodstream infection (BSI) refers to the infection of blood by pathogens. Severe immune response to BSI can lead to sepsis, a systemic infection leading to multiple organ dysfunction, coupled with drug resistance, mortality, and limited clinical treatment options. This work aims to further investigate the new interplay between bacterial exocrine regulatory protein and host immune cells in the context of highly drug-resistant malignant BSI. Whether interfering with related regulatory signaling pathways can reverse the inflammatory disorder of immune cells. In-depth analysis of single-cell sequencing results in Septic patients for potential immunodeficiency factors. Analysis of key proteins enriched by host cells and key pathways using proteomics. Cell models and animal models validate the pathological effects of DnaK on T cells, MAITs, macrophages, and osteoclasts. The blood of patients was analyzed for the immunosuppression of T cells and MAITs. We identified that S. maltophilia-DnaK was enriched in immunodeficient T cells. The activation of the JAK2/STAT1 axis initiated the exhaustion of T cells. Septic patients with Gram-negative bacterial infections exhibited deficiencies in MAITs, which correspond to IFN-γ. Cellular and animal experiments confirmed that DnaK could facilitate MAIT depletion and M1 polarization of macrophages. Additionally, Fludarabine mitigated M1 polarization of blood, liver, and spleen in mice. Interestingly, DnaK also repressed osteoclastogenesis of macrophages stimulated by RANKL. S.maltophilia-DnaK prompts the activation of the JAK2/STAT1 axis in T cells and the M1 polarization of macrophages. Targeting the DnaK's crosstalk can be a potentially effective approach for treating the inflammatory disorder in the broad-spectrum drug-resistant BSI | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Bloodstream infection | |
| 650 | 4 | |a Fludarabine | |
| 650 | 4 | |a Macrophages | |
| 650 | 4 | |a Mucosa-associated invariant T | |
| 650 | 4 | |a Stenotrophomonas maltophilia | |
| 650 | 7 | |a Heat-Shock Proteins |2 NLM | |
| 650 | 7 | |a Anti-Infective Agents |2 NLM | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
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| 650 | 7 | |a STAT1 Transcription Factor |2 NLM | |
| 650 | 7 | |a JAK2 protein, human |2 NLM | |
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| 700 | 1 | |a Peng, Yizhi |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, YingRui |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Hongzheng |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Bingqi |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiaofan |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Wanxin |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Tianyao |e verfasserin |4 aut | |
| 700 | 1 | |a Peng, Huanqie |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xianping |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Jiake |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Min |e verfasserin |4 aut | |
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