B cell immune response to human bocaviruses
© 2024 The Authors. Clinical & Experimental Allergy published by John Wiley & Sons Ltd..
BACKGROUND: Human bocaviruses (HBoVs) have been demonstrated in respiratory and gastrointestinal infections; however, the immune response to them has not been studied in detail. In this study, we investigated the B cell immune responses to HBoV1 and HBoV2, representing two different species of bocaviruses in humans.
METHODS: We analyzed the effects of stimulations with HBoV1 and 2 virus-like particles (VLPs) and of co-stimulation with HBoV1-rhinovirus (RV) on cells of the immune system by flow cytometry, transcriptomics, and luminometric immune assays.
RESULTS: Human B cells, and particularly B regulatory cells (Breg cells), showed an increased immune response to HBoV1-VLPs stimulation. These immune responses were also supported by increased IL-1RA and PDL1 expressions in IL-10+ B cells from peripheral blood mononuclear cells (PBMCs) stimulated with HBoV1-VLPs. In addition, increased levels of IL-10 and IL-1RA were determined in the supernatants of PBMCs following HBoV1-VLPs stimulation. HBoV1-VLPs and RV co-stimulation increased the IL-10+ B cell population. Transcriptome analysis by next-generation RNA sequencing showed an increased expression of IL-10 signalling and Breg cell markers in PBMCs stimulated with HBoV1-VLPs. Furthermore, TGF-β and chemoattractants MIP-1α, MIP-1β and IP10 protein levels were high in the supernatants of PBMCs stimulated with HBoV1-VLPs.
CONCLUSIONS: The findings demonstrate that in Breg cells, IL-10 signalling pathways, and anti-inflammatory activity are induced by HBoV1, which can explain the often mild nature of the disease. In addition, the immune regulatory response induced by HBoV1-VLPs may indicate a potential immunomodulatory role of HBoV1 on the immune system and may represent an immune regulatory strategy.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology - (2024) vom: 06. Feb. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Karaaslan, Cagatay [VerfasserIn] |
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Date Revised 07.02.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1111/cea.14453 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM368109259 |
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| 520 | |a © 2024 The Authors. Clinical & Experimental Allergy published by John Wiley & Sons Ltd. | ||
| 520 | |a BACKGROUND: Human bocaviruses (HBoVs) have been demonstrated in respiratory and gastrointestinal infections; however, the immune response to them has not been studied in detail. In this study, we investigated the B cell immune responses to HBoV1 and HBoV2, representing two different species of bocaviruses in humans | ||
| 520 | |a METHODS: We analyzed the effects of stimulations with HBoV1 and 2 virus-like particles (VLPs) and of co-stimulation with HBoV1-rhinovirus (RV) on cells of the immune system by flow cytometry, transcriptomics, and luminometric immune assays | ||
| 520 | |a RESULTS: Human B cells, and particularly B regulatory cells (Breg cells), showed an increased immune response to HBoV1-VLPs stimulation. These immune responses were also supported by increased IL-1RA and PDL1 expressions in IL-10+ B cells from peripheral blood mononuclear cells (PBMCs) stimulated with HBoV1-VLPs. In addition, increased levels of IL-10 and IL-1RA were determined in the supernatants of PBMCs following HBoV1-VLPs stimulation. HBoV1-VLPs and RV co-stimulation increased the IL-10+ B cell population. Transcriptome analysis by next-generation RNA sequencing showed an increased expression of IL-10 signalling and Breg cell markers in PBMCs stimulated with HBoV1-VLPs. Furthermore, TGF-β and chemoattractants MIP-1α, MIP-1β and IP10 protein levels were high in the supernatants of PBMCs stimulated with HBoV1-VLPs | ||
| 520 | |a CONCLUSIONS: The findings demonstrate that in Breg cells, IL-10 signalling pathways, and anti-inflammatory activity are induced by HBoV1, which can explain the often mild nature of the disease. In addition, the immune regulatory response induced by HBoV1-VLPs may indicate a potential immunomodulatory role of HBoV1 on the immune system and may represent an immune regulatory strategy | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a B cells | |
| 650 | 4 | |a Breg cells | |
| 650 | 4 | |a HBoV1 | |
| 650 | 4 | |a IL-10 signalling | |
| 650 | 4 | |a PBMCs | |
| 700 | 1 | |a Wirz, Oliver |e verfasserin |4 aut | |
| 700 | 1 | |a Tan, Ge |e verfasserin |4 aut | |
| 700 | 1 | |a Globinska, Anna |e verfasserin |4 aut | |
| 700 | 1 | |a Boonpiyathad, Tadech |e verfasserin |4 aut | |
| 700 | 1 | |a Hedman, Klaus |e verfasserin |4 aut | |
| 700 | 1 | |a Vaselek, Slavica |e verfasserin |4 aut | |
| 700 | 1 | |a Venermo, Maria Söderlund |e verfasserin |4 aut | |
| 700 | 1 | |a Jartti, Tuomas |e verfasserin |4 aut | |
| 700 | 1 | |a Akdis, Mubeccel |e verfasserin |4 aut | |
| 700 | 1 | |a Akdis, Cezmi A |e verfasserin |4 aut | |
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