Detecting Hypoxia-Inducible Factor Levels and Activity
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
The hypoxia-inducible transcription factors HIF-1 and HIF-2 regulate the response to hypoxia. Both proteins are dimers of an alpha subunit and a shared beta subunit. Under hypoxic conditions, the alpha subunits are stabilized, and the transactivation ability of the HIF-1 transcription factor is induced. Accordingly, assessment of HIF-1α protein levels and HIF transcriptional activity serve as an indirect indicator of hypoxia. In this series of protocols, I describe three methods to probe the HIF pathway.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2755 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2755(2024) vom: 06., Seite 179-187 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hubbi, Maimon E [VerfasserIn] |
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| Links: |
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| Themen: |
Hypoxia |
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| Anmerkungen: |
Date Completed 07.02.2024 Date Revised 07.02.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-3633-6_13 |
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| 520 | |a The hypoxia-inducible transcription factors HIF-1 and HIF-2 regulate the response to hypoxia. Both proteins are dimers of an alpha subunit and a shared beta subunit. Under hypoxic conditions, the alpha subunits are stabilized, and the transactivation ability of the HIF-1 transcription factor is induced. Accordingly, assessment of HIF-1α protein levels and HIF transcriptional activity serve as an indirect indicator of hypoxia. In this series of protocols, I describe three methods to probe the HIF pathway | ||
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