Crocin-loaded Niosomal Nanoparticles Reversing Cytotoxicity and Oxidative Stress in HEK293 Cell Line Exposed to Paraquat : An In vitro Study
Copyright© Bentham Science Publishers; For any queries, please email at epubbenthamscience.net..
BACKGROUND: Paraquat (PQ) is an effective herbicide which is widely used around the world to remove weeds in agriculture. As a water-soluble carotenoid, crocin is a pharmacologically active constituent of C. sativus L. (saffron).
OBJECTIVES: In the present study, we investigated the effects of crocin-loaded niosomes (Cro-NIO) compared to free crocin on PQ-induced toxicity in the eukaryotic human embryonic kidney (HEK293) cell line.
METHODS: The Cro-NIO was synthesized and characterized. Cell viability was determined using the MTT assay in PQ-exposed HEK293 cell lines. The activities of biochemical markers were quantitatively determined to reveal the potential mechanism of PQ-induced oxidative stress in HEK293 cell line.
RESULTS: The particle size, zeta potential, polydispersity index (PDI), DL, and EE of Cro-NIO were 145.4 ± 19.5 nm, -22.3 ± 3.11 mV, 0.3 ± 0.03, 1.74 ± 0.01%, and 55.3 ± 7.1%, respectively. PQtreated HEK293 cell lines decreased cell viability. The results of oxidative status showed that PQ significantly could increase ROS accumulation, accompanied by a decreasing antioxidant defense system. However, treatment with Cro-NIO, compared to crocin, not only did dose-dependently improve the cell viability but also significantly attenuated the ROS accumulation and increased antioxidant markers.
CONCLUSION: According to these results, Cro-NIO, compared to crocin, was superior to ameliorating PQ-induced cytotoxicity and oxidative damage in HEK293 cells.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
Pharmaceutical nanotechnology - (2024) vom: 16. Jan. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Oftadeh Harsin, Akram [VerfasserIn] |
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| Links: |
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| Themen: |
Cell lines |
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| Anmerkungen: |
Date Revised 06.02.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.2174/0122117385256493231019045141 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM368067297 |
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| 500 | |a Date Revised 06.02.2024 | ||
| 500 | |a published: Print-Electronic | ||
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| 520 | |a Copyright© Bentham Science Publishers; For any queries, please email at epubbenthamscience.net. | ||
| 520 | |a BACKGROUND: Paraquat (PQ) is an effective herbicide which is widely used around the world to remove weeds in agriculture. As a water-soluble carotenoid, crocin is a pharmacologically active constituent of C. sativus L. (saffron) | ||
| 520 | |a OBJECTIVES: In the present study, we investigated the effects of crocin-loaded niosomes (Cro-NIO) compared to free crocin on PQ-induced toxicity in the eukaryotic human embryonic kidney (HEK293) cell line | ||
| 520 | |a METHODS: The Cro-NIO was synthesized and characterized. Cell viability was determined using the MTT assay in PQ-exposed HEK293 cell lines. The activities of biochemical markers were quantitatively determined to reveal the potential mechanism of PQ-induced oxidative stress in HEK293 cell line | ||
| 520 | |a RESULTS: The particle size, zeta potential, polydispersity index (PDI), DL, and EE of Cro-NIO were 145.4 ± 19.5 nm, -22.3 ± 3.11 mV, 0.3 ± 0.03, 1.74 ± 0.01%, and 55.3 ± 7.1%, respectively. PQtreated HEK293 cell lines decreased cell viability. The results of oxidative status showed that PQ significantly could increase ROS accumulation, accompanied by a decreasing antioxidant defense system. However, treatment with Cro-NIO, compared to crocin, not only did dose-dependently improve the cell viability but also significantly attenuated the ROS accumulation and increased antioxidant markers | ||
| 520 | |a CONCLUSION: According to these results, Cro-NIO, compared to crocin, was superior to ameliorating PQ-induced cytotoxicity and oxidative damage in HEK293 cells | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Crocin | |
| 650 | 4 | |a Niosome | |
| 650 | 4 | |a Oxidative stress | |
| 650 | 4 | |a Paraquat | |
| 650 | 4 | |a cell lines | |
| 650 | 4 | |a human embryonic kidney (HEK293) cell | |
| 700 | 1 | |a Makhdoomi, Sajjad |e verfasserin |4 aut | |
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| 700 | 1 | |a Firozian, Farzin |e verfasserin |4 aut | |
| 700 | 1 | |a Nili-Ahmadabadi, Amir |e verfasserin |4 aut | |
| 700 | 1 | |a Ranjbar, Akram |e verfasserin |4 aut | |
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