Lithocholic acid-based oligomers as drug delivery candidates targeting model of lipid raft
Copyright © 2024 Elsevier B.V. All rights reserved..
This study presents a new approach to designing a lithocholic acid functionalized oligomer (OLithocholicAA-X) that can be used as a drug carrier with additional, beneficial activity. Namely, this novel oligomer can incorporate an anti-cancer drug due to the application of an effective backbone as its component (lithocholic acid) alone is known to have anticancer activity. The oligomer was synthesized and characterized in detail by nuclear magnetic resonance, attenuated total reflectance Fourier-transform infrared spectroscopy, ultraviolet-visible spectroscopy, thermal analysis, and mass spectrometry analysis. We selected lipid rafts as potential drug carrier-membrane binding sites. In this respect, we investigated the effects of OLithocholicAA-X on model lipid raft of normal and altered composition, containing an increased amount of cholesterol (Chol) or sphingomyelin (SM), using Langmuir monolayers and liposomes. The surface topography of the studied monolayers was additionally investigated by atomic force microscopy (AFM). The obtained results showed that the investigated oligomer has affinity for a system that mimics a normal lipid raft (SM:Chol 2:1). On the other hand, for systems with an excess of SM or Chol, thermodynamically unfavorable fluidization of the films occurs. Moreover, AFM topographies showed that the amount of SM determines the bioavailability of the oligomer, causing fragmentation of its lattice.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1866 |
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| Enthalten in: |
Biochimica et biophysica acta. Biomembranes - 1866(2024), 3 vom: 25. Feb., Seite 184294 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wnętrzak, Anita [VerfasserIn] |
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| Links: |
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| Themen: |
5QU0I8393U |
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| Anmerkungen: |
Date Completed 21.02.2024 Date Revised 21.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bbamem.2024.184294 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM368056430 |
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| 245 | 1 | 0 | |a Lithocholic acid-based oligomers as drug delivery candidates targeting model of lipid raft |
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| 520 | |a This study presents a new approach to designing a lithocholic acid functionalized oligomer (OLithocholicAA-X) that can be used as a drug carrier with additional, beneficial activity. Namely, this novel oligomer can incorporate an anti-cancer drug due to the application of an effective backbone as its component (lithocholic acid) alone is known to have anticancer activity. The oligomer was synthesized and characterized in detail by nuclear magnetic resonance, attenuated total reflectance Fourier-transform infrared spectroscopy, ultraviolet-visible spectroscopy, thermal analysis, and mass spectrometry analysis. We selected lipid rafts as potential drug carrier-membrane binding sites. In this respect, we investigated the effects of OLithocholicAA-X on model lipid raft of normal and altered composition, containing an increased amount of cholesterol (Chol) or sphingomyelin (SM), using Langmuir monolayers and liposomes. The surface topography of the studied monolayers was additionally investigated by atomic force microscopy (AFM). The obtained results showed that the investigated oligomer has affinity for a system that mimics a normal lipid raft (SM:Chol 2:1). On the other hand, for systems with an excess of SM or Chol, thermodynamically unfavorable fluidization of the films occurs. Moreover, AFM topographies showed that the amount of SM determines the bioavailability of the oligomer, causing fragmentation of its lattice | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Artificial membrane models | |
| 650 | 4 | |a Drug carriers | |
| 650 | 4 | |a Drug incorporation | |
| 650 | 4 | |a Langmuir monolayers | |
| 650 | 4 | |a Lipid rafts | |
| 650 | 4 | |a Liposomes | |
| 650 | 7 | |a Lithocholic Acid |2 NLM | |
| 650 | 7 | |a 5QU0I8393U |2 NLM | |
| 650 | 7 | |a Liposomes |2 NLM | |
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| 700 | 1 | |a Szymczuk, Dawid |e verfasserin |4 aut | |
| 700 | 1 | |a Chachaj-Brekiesz, Anna |e verfasserin |4 aut | |
| 700 | 1 | |a Dynarowicz-Latka, Patrycja |e verfasserin |4 aut | |
| 700 | 1 | |a Lupa, Dawid |e verfasserin |4 aut | |
| 700 | 1 | |a Lipiec, Ewelina W |e verfasserin |4 aut | |
| 700 | 1 | |a Laszuk, Paulina |e verfasserin |4 aut | |
| 700 | 1 | |a Petelska, Aneta D |e verfasserin |4 aut | |
| 700 | 1 | |a Markiewicz, Karolina H |e verfasserin |4 aut | |
| 700 | 1 | |a Wilczewska, Agnieszka Z |e verfasserin |4 aut | |
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