Immunochromatographic visualization detection platform for bitertanol in foods
Copyright © 2024 Elsevier Ltd. All rights reserved..
As a widely used fungicide in agriculture, bitertanol (BIT) significantly affects hormone regulation leading to imbalance of homeostasis in vivo, which makes it necessary to monitor BIT residues in foods. In this research, a novel hapten derivation scheme was designed by analyzing the chemical structure of BIT to prepare an anti-BIT monoclonal antibody with high affinity, specificity and sensitivity (half inhibitory concentration of 4.78 ng/mL). Subsequently, a visualized gold immunochromatographic assay (GICA) platform was established based on antigen-antibody specific recognition, with a limit of detection of 0.06 mg/kg and 0.18 mg/kg in cucumber and tomato, respectively. GICA has spiked recoveries of 84.3 %-114.1 %, determines results are not significantly different from those of LC-MS/MS, and the complex purification treatments can be reduced during the detection process. Therefore, the developed GICA is a reliable, rapid, and sensitive method for on-site rapid monitoring of BIT in foods.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:444 |
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| Enthalten in: |
Food chemistry - 444(2024) vom: 30. März, Seite 138599 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Yang [VerfasserIn] |
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| Links: |
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| Themen: |
7440-57-5 |
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| Anmerkungen: |
Date Completed 11.03.2024 Date Revised 11.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.foodchem.2024.138599 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM368002810 |
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| 520 | |a Copyright © 2024 Elsevier Ltd. All rights reserved. | ||
| 520 | |a As a widely used fungicide in agriculture, bitertanol (BIT) significantly affects hormone regulation leading to imbalance of homeostasis in vivo, which makes it necessary to monitor BIT residues in foods. In this research, a novel hapten derivation scheme was designed by analyzing the chemical structure of BIT to prepare an anti-BIT monoclonal antibody with high affinity, specificity and sensitivity (half inhibitory concentration of 4.78 ng/mL). Subsequently, a visualized gold immunochromatographic assay (GICA) platform was established based on antigen-antibody specific recognition, with a limit of detection of 0.06 mg/kg and 0.18 mg/kg in cucumber and tomato, respectively. GICA has spiked recoveries of 84.3 %-114.1 %, determines results are not significantly different from those of LC-MS/MS, and the complex purification treatments can be reduced during the detection process. Therefore, the developed GICA is a reliable, rapid, and sensitive method for on-site rapid monitoring of BIT in foods | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Bitertanol | |
| 650 | 4 | |a Cucumber | |
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| 650 | 4 | |a Immunoassay | |
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| 700 | 1 | |a Liu, Liqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Chuanlai |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Maozhong |e verfasserin |4 aut | |
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