Modulation of gut microbiota by crude mulberry polysaccharide attenuates knee osteoarthritis progression in rats
Copyright © 2024. Published by Elsevier B.V..
Mulberry (Morus alba L.), a kind of common fruits widely cultivated worldwide, has been proven various biological activities. However, its potential role in the progression of knee osteoarthritis (KOA) remains unclear. This study aims to investigate the potential protective effects of crude polysaccharide extracted from mulberry fruit, referred to as a complex blend of polysaccharides and other unidentified extracted impurities, on KOA progression. The KOA rats were established by injection of 1 mg sodium monoiodoacetate into knee, and administrated with crude mulberry polysaccharide (Mup) by gastric gavage for 4 weeks. Furthermore, intestinal bacteria clearance assay (IBCA) and fecal microbiota transplantation were conducted for the evaluation of the effect of gut microbiota (GM) on KOA. Our findings demonstrated that Mup, particularly at a dosage of 200 mg/kg, effectively improved abnormal gait patterns, reduced the level of inflammation, mitigated subchondral bone loss, restored compromised joint surfaces, alleviated cartilage destruction, and positively modulated the dysregulated profile of GM in KOA rats. Moreover, IBCA compromised the protective effects of Mup, while transplantation of fecal bacteria from Mup-treated rats facilitated KOA recovery. Collectively, our study suggested that Mup had the potential to ameliorate the progression of KOA, potentially through its modulation of GM profile.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:262 |
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| Enthalten in: |
International journal of biological macromolecules - 262(2024), Pt 2 vom: 27. März, Seite 129936 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zheng, Yi-Zhou [VerfasserIn] |
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| Links: |
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| Themen: |
Gut microbiota |
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| Anmerkungen: |
Date Completed 27.03.2024 Date Revised 27.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ijbiomac.2024.129936 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM367988682 |
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| 245 | 1 | 0 | |a Modulation of gut microbiota by crude mulberry polysaccharide attenuates knee osteoarthritis progression in rats |
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| 520 | |a Copyright © 2024. Published by Elsevier B.V. | ||
| 520 | |a Mulberry (Morus alba L.), a kind of common fruits widely cultivated worldwide, has been proven various biological activities. However, its potential role in the progression of knee osteoarthritis (KOA) remains unclear. This study aims to investigate the potential protective effects of crude polysaccharide extracted from mulberry fruit, referred to as a complex blend of polysaccharides and other unidentified extracted impurities, on KOA progression. The KOA rats were established by injection of 1 mg sodium monoiodoacetate into knee, and administrated with crude mulberry polysaccharide (Mup) by gastric gavage for 4 weeks. Furthermore, intestinal bacteria clearance assay (IBCA) and fecal microbiota transplantation were conducted for the evaluation of the effect of gut microbiota (GM) on KOA. Our findings demonstrated that Mup, particularly at a dosage of 200 mg/kg, effectively improved abnormal gait patterns, reduced the level of inflammation, mitigated subchondral bone loss, restored compromised joint surfaces, alleviated cartilage destruction, and positively modulated the dysregulated profile of GM in KOA rats. Moreover, IBCA compromised the protective effects of Mup, while transplantation of fecal bacteria from Mup-treated rats facilitated KOA recovery. Collectively, our study suggested that Mup had the potential to ameliorate the progression of KOA, potentially through its modulation of GM profile | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Gut microbiota | |
| 650 | 4 | |a Knee osteoarthritis | |
| 650 | 4 | |a Mulberry polysaccharide | |
| 650 | 7 | |a Polysaccharides |2 NLM | |
| 700 | 1 | |a Chen, Qing-Rou |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Hong-Mei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Ji-Ao |e verfasserin |4 aut | |
| 700 | 1 | |a Ren, Ling-Zhi |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Ye-Qun |e verfasserin |4 aut | |
| 700 | 1 | |a Long, Yong-Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Tong-Ming |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Yang |e verfasserin |4 aut | |
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