Pyramiding D-lactate dehydrogenase with the glyoxalase pathway enhances abiotic stress tolerance in plants
Copyright © 2024 Elsevier Masson SAS. All rights reserved..
Methylglyoxal is a common cytotoxic metabolite produced in plants during multiple biotic and abiotic stress. To mitigate the toxicity of MG, plants utilize the glyoxalase pathway comprising glyoxalase I (GLYI), glyoxalase II (GLYII), or glyoxalase III (GLYIII). GLYI and GLYII are the key enzymes of glyoxalase pathways that play an important role in abiotic stress tolerance. Earlier research showed that MG level is lower when both GLYI and GLYII are overexpressed together, compared to GLYI or GLYII single gene overexpressed transgenic plants. D-lactate dehydrogenase (D-LDH) is an integral part of MG detoxification which metabolizes the end product (D-lactate) of the glyoxalase pathway. In this study, two Arabidopsis transgenic lines were constructed using gene pyramiding technique: GLYI and GLYII overexpressed (G-I + II), and GLYI, GLYII, and D-LDH overexpressed (G-I + II + D) plants. G-I + II + D exhibits lower MG and D-lactate levels and enhanced abiotic stress tolerance than the G-I + II and wild-type plants. Further study explores the stress tolerance mechanism of G-I + II + D plants through the interplay of different regulators and plant hormones. This, in turn, modulates the expression of ABA-dependent stress-responsive genes like RAB18, RD22, and RD29B to generate adaptive responses during stress. Therefore, there might be a potential correlation between ABA and MG detoxification pathways. Furthermore, higher STY46, GPX3, and CAMTA1 transcripts were observed in G-I + II + D plants during abiotic stress. Thus, our findings suggest that G-I + II + D has significantly improved MG detoxification, reduced oxidative stress-induced damage, and provided a better protective mechanism against abiotic stresses than G-I + II or wild-type plants.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:207 |
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| Enthalten in: |
Plant physiology and biochemistry : PPB - 207(2024) vom: 03. Feb., Seite 108391 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Alam, Nazmir Binta [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 18.03.2024 Date Revised 18.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.plaphy.2024.108391 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Methylglyoxal is a common cytotoxic metabolite produced in plants during multiple biotic and abiotic stress. To mitigate the toxicity of MG, plants utilize the glyoxalase pathway comprising glyoxalase I (GLYI), glyoxalase II (GLYII), or glyoxalase III (GLYIII). GLYI and GLYII are the key enzymes of glyoxalase pathways that play an important role in abiotic stress tolerance. Earlier research showed that MG level is lower when both GLYI and GLYII are overexpressed together, compared to GLYI or GLYII single gene overexpressed transgenic plants. D-lactate dehydrogenase (D-LDH) is an integral part of MG detoxification which metabolizes the end product (D-lactate) of the glyoxalase pathway. In this study, two Arabidopsis transgenic lines were constructed using gene pyramiding technique: GLYI and GLYII overexpressed (G-I + II), and GLYI, GLYII, and D-LDH overexpressed (G-I + II + D) plants. G-I + II + D exhibits lower MG and D-lactate levels and enhanced abiotic stress tolerance than the G-I + II and wild-type plants. Further study explores the stress tolerance mechanism of G-I + II + D plants through the interplay of different regulators and plant hormones. This, in turn, modulates the expression of ABA-dependent stress-responsive genes like RAB18, RD22, and RD29B to generate adaptive responses during stress. Therefore, there might be a potential correlation between ABA and MG detoxification pathways. Furthermore, higher STY46, GPX3, and CAMTA1 transcripts were observed in G-I + II + D plants during abiotic stress. Thus, our findings suggest that G-I + II + D has significantly improved MG detoxification, reduced oxidative stress-induced damage, and provided a better protective mechanism against abiotic stresses than G-I + II or wild-type plants | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a ABA | |
| 650 | 4 | |a Abiotic stress | |
| 650 | 4 | |a D-lactate dehydrogenase | |
| 650 | 4 | |a Gene Pyramiding | |
| 650 | 4 | |a Glyoxalase I | |
| 650 | 4 | |a Glyoxalase II | |
| 650 | 4 | |a MG | |
| 650 | 4 | |a Methylglyoxal | |
| 650 | 4 | |a ROS | |
| 650 | 7 | |a Lactoylglutathione Lyase |2 NLM | |
| 650 | 7 | |a EC 4.4.1.5 |2 NLM | |
| 650 | 7 | |a D-lactate dehydrogenase |2 NLM | |
| 650 | 7 | |a EC 1.1.1.28 |2 NLM | |
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| 650 | 7 | |a Lactates |2 NLM | |
| 650 | 7 | |a Pyruvaldehyde |2 NLM | |
| 650 | 7 | |a 722KLD7415 |2 NLM | |
| 650 | 7 | |a GPX3 protein, Arabidopsis |2 NLM | |
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| 650 | 7 | |a Glutathione Peroxidase |2 NLM | |
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| 650 | 7 | |a Arabidopsis Proteins |2 NLM | |
| 650 | 7 | |a Lactate Dehydrogenases |2 NLM | |
| 650 | 7 | |a EC 1.1.- |2 NLM | |
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| 700 | 1 | |a Mustafiz, Ananda |e verfasserin |4 aut | |
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