Intestinal helminth infection impairs vaccine-induced T cell responses and protection against SARS-CoV-2
Although vaccines have reduced COVID-19 disease burden, their efficacy in helminth infection endemic areas is not well characterized. We evaluated the impact of infection by Heligmosomoides polygyrus bakeri (Hpb), a murine intestinal hookworm, on the efficacy of an mRNA vaccine targeting the Wuhan-1 spike protein of SARS-CoV-2. Although immunization generated similar B cell responses in Hpb-infected and uninfected mice, polyfunctional CD4+ and CD8+ T cell responses were markedly reduced in Hpb-infected mice. Hpb-infected and mRNA vaccinated mice were protected against the ancestral SARS-CoV-2 strain WA1/2020, but control of lung infection was diminished against an Omicron variant compared to animals immunized without Hpb infection. Helminth mediated suppression of spike-specific CD8+ T cell responses occurred independently of STAT6 signaling, whereas blockade of IL-10 rescued vaccine-induced CD8+ T cell responses. In mice, intestinal helminth infection impairs vaccine induced T cell responses via an IL-10 pathway and compromises protection against antigenically shifted SARS-CoV-2 variants.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2024 |
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Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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Enthalten in: |
bioRxiv : the preprint server for biology - (2024) vom: 15. Jan. |
Sprache: |
Englisch |
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Beteiligte Personen: |
Desai, Pritesh [VerfasserIn] |
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Anmerkungen: |
Date Revised 05.02.2024 published: Electronic Citation Status PubMed-not-MEDLINE |
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doi: |
10.1101/2024.01.14.575588 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM367835371 |
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520 | |a Although vaccines have reduced COVID-19 disease burden, their efficacy in helminth infection endemic areas is not well characterized. We evaluated the impact of infection by Heligmosomoides polygyrus bakeri (Hpb), a murine intestinal hookworm, on the efficacy of an mRNA vaccine targeting the Wuhan-1 spike protein of SARS-CoV-2. Although immunization generated similar B cell responses in Hpb-infected and uninfected mice, polyfunctional CD4+ and CD8+ T cell responses were markedly reduced in Hpb-infected mice. Hpb-infected and mRNA vaccinated mice were protected against the ancestral SARS-CoV-2 strain WA1/2020, but control of lung infection was diminished against an Omicron variant compared to animals immunized without Hpb infection. Helminth mediated suppression of spike-specific CD8+ T cell responses occurred independently of STAT6 signaling, whereas blockade of IL-10 rescued vaccine-induced CD8+ T cell responses. In mice, intestinal helminth infection impairs vaccine induced T cell responses via an IL-10 pathway and compromises protection against antigenically shifted SARS-CoV-2 variants | ||
650 | 4 | |a Preprint | |
700 | 1 | |a Karl, Courtney E |e verfasserin |4 aut | |
700 | 1 | |a Ying, Baoling |e verfasserin |4 aut | |
700 | 1 | |a Liang, Chieh-Yu |e verfasserin |4 aut | |
700 | 1 | |a Garcia-Salum, Tamara |e verfasserin |4 aut | |
700 | 1 | |a Santana, Ana Carolina |e verfasserin |4 aut | |
700 | 1 | |a Caten, Felipe Ten |e verfasserin |4 aut | |
700 | 1 | |a Urban, Joseph F |c Jr |e verfasserin |4 aut | |
700 | 1 | |a Elbashir, Sayda M |e verfasserin |4 aut | |
700 | 1 | |a Edwards, Darin K |e verfasserin |4 aut | |
700 | 1 | |a Ribeiro, Susan P |e verfasserin |4 aut | |
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700 | 1 | |a Sekaly, Rafick P |e verfasserin |4 aut | |
700 | 1 | |a Diamond, Michael S |e verfasserin |4 aut | |
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