Monitoring of hepatitis E virus in wastewater can identify clinically relevant variants
© 2024 The Authors. Liver International published by John Wiley & Sons Ltd..
Hepatitis E virus (HEV) is prevalent worldwide and can cause persistent infection with severe morbidity. Antiviral treatment approaches can lead to the emergence of viral variants encoding escape mutations that may impede viral clearance. The frequency of these variants remains unknown in the human population as well as environment due to limited comprehensive data on HEV diversity. In this study, we investigated the HEV prevalence and diversity of circulating variants in environmental samples, that is, wastewater and rivers from North-Rhine Westphalia, Germany. HEV prevalence could be determined with 73% of samples tested positive for viral RNA via qRT-PCR. Using high-throughput sequencing, we were able to assess the overall genetic diversity in these samples and identified the presence of clinically relevant variants associated with drug resistance. In summary, monitoring variants from environmental samples could provide valuable insights into estimating HEV prevalence and identifying circulating variants that can impact treatment outcome.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:44 |
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| Enthalten in: |
Liver international : official journal of the International Association for the Study of the Liver - 44(2024), 3 vom: 31. März, Seite 637-643 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Rau, Fiona [VerfasserIn] |
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| Links: |
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| Themen: |
Hepatitis E virus |
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| Anmerkungen: |
Date Completed 01.03.2024 Date Revised 01.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/liv.15842 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM367821664 |
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| 520 | |a © 2024 The Authors. Liver International published by John Wiley & Sons Ltd. | ||
| 520 | |a Hepatitis E virus (HEV) is prevalent worldwide and can cause persistent infection with severe morbidity. Antiviral treatment approaches can lead to the emergence of viral variants encoding escape mutations that may impede viral clearance. The frequency of these variants remains unknown in the human population as well as environment due to limited comprehensive data on HEV diversity. In this study, we investigated the HEV prevalence and diversity of circulating variants in environmental samples, that is, wastewater and rivers from North-Rhine Westphalia, Germany. HEV prevalence could be determined with 73% of samples tested positive for viral RNA via qRT-PCR. Using high-throughput sequencing, we were able to assess the overall genetic diversity in these samples and identified the presence of clinically relevant variants associated with drug resistance. In summary, monitoring variants from environmental samples could provide valuable insights into estimating HEV prevalence and identifying circulating variants that can impact treatment outcome | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a hepatitis E virus | |
| 650 | 4 | |a surveillance | |
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| 650 | 4 | |a wastewater-based epidemiology | |
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| 700 | 1 | |a Elsner, Carina |e verfasserin |4 aut | |
| 700 | 1 | |a Meister, Toni Luise |e verfasserin |4 aut | |
| 700 | 1 | |a Gömer, André |e verfasserin |4 aut | |
| 700 | 1 | |a Kallies, René |e verfasserin |4 aut | |
| 700 | 1 | |a Dittmer, Ulf |e verfasserin |4 aut | |
| 700 | 1 | |a Steinmann, Eike |e verfasserin |4 aut | |
| 700 | 1 | |a Todt, Daniel |e verfasserin |4 aut | |
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