Structural characterization and anti-oxidative activity for a glycopeptide from Ganoderma lucidum fruiting body
Copyright © 2024 Elsevier B.V. All rights reserved..
A water-soluble glycopeptide (named GL-PWQ3) with a molecular weight (Mw) of 2.40 × 104 g/mol was isolated from Ganoderma lucidum fruiting body by hot water extraction, membrane ultrafiltration, and gel column chromatography, which mainly consisted of glucose and galactose. Based on the methylation, FT-IR, 1D, and 2D NMR analysis, the polysaccharide portion of GL-PWQ3 was identified as a glucogalactan, which was comprised of unsubstituted (1,6-α-Galp, 1,6-β-Glcp, 1,4-β-Glcp) and monosubstituted (1,2,6-α-Galp and 1,3,6-β-Glcp) in the backbone and possible branches that at the O-3 position of 1,3-Glcp and T-Glcp, and the O-2 position of T-Fucp, T-Manp or T-Glcp. The chain conformational study by SEC-MALLS-RI and AFM revealed that GL-PWQ3 was identified as a highly branched polysaccharide with a polydispersity index of 1.25, and might have compact sphere structures caused by stacked multiple chains. Moreover, the GL-PWQ3 shows strong anti-oxidative activity in NRK-52E cells. This study provides a theoretical basis for further elucidating the structure-functionality relationships of GL-PWQ3 and its potential application as a natural antioxidant in pharmacotherapy as well as functional food additives.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:261 |
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| Enthalten in: |
International journal of biological macromolecules - 261(2024), Pt 2 vom: 11. März, Seite 129793 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Luo, Hong-Jian [VerfasserIn] |
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| Links: |
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| Themen: |
059QF0KO0R |
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| Anmerkungen: |
Date Completed 13.03.2024 Date Revised 13.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ijbiomac.2024.129793 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM367809451 |
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| 245 | 1 | 0 | |a Structural characterization and anti-oxidative activity for a glycopeptide from Ganoderma lucidum fruiting body |
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| 520 | |a Copyright © 2024 Elsevier B.V. All rights reserved. | ||
| 520 | |a A water-soluble glycopeptide (named GL-PWQ3) with a molecular weight (Mw) of 2.40 × 104 g/mol was isolated from Ganoderma lucidum fruiting body by hot water extraction, membrane ultrafiltration, and gel column chromatography, which mainly consisted of glucose and galactose. Based on the methylation, FT-IR, 1D, and 2D NMR analysis, the polysaccharide portion of GL-PWQ3 was identified as a glucogalactan, which was comprised of unsubstituted (1,6-α-Galp, 1,6-β-Glcp, 1,4-β-Glcp) and monosubstituted (1,2,6-α-Galp and 1,3,6-β-Glcp) in the backbone and possible branches that at the O-3 position of 1,3-Glcp and T-Glcp, and the O-2 position of T-Fucp, T-Manp or T-Glcp. The chain conformational study by SEC-MALLS-RI and AFM revealed that GL-PWQ3 was identified as a highly branched polysaccharide with a polydispersity index of 1.25, and might have compact sphere structures caused by stacked multiple chains. Moreover, the GL-PWQ3 shows strong anti-oxidative activity in NRK-52E cells. This study provides a theoretical basis for further elucidating the structure-functionality relationships of GL-PWQ3 and its potential application as a natural antioxidant in pharmacotherapy as well as functional food additives | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Anti-oxidant activity | |
| 650 | 4 | |a Ganoderma lucidum glycopeptide | |
| 650 | 4 | |a Physicochemical property | |
| 650 | 4 | |a Structural characterization | |
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| 700 | 1 | |a Lin, Shu-Qian |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Lian-Fu |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Zhan-Xi |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Guo-Dong |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Dong-Mei |e verfasserin |4 aut | |
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