Study on the preparation and enzyme inhibitory activity of polyphenols from Sargassum pallidum
Copyright: © 2024 Jiang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited..
This study aimed to obtain a high yield and purity of Sargassum pallidum polyphenol extracts (SPPE) and study its enzyme activity. Fresh Sargassum pallidum seaweed was selected for optimization of ultrasound-assisted extraction (UAE) conditions and purification conditions using macroporous resin and Sephadex LH20 to obtain SPPE. The SPPE was characterized using UPLC-QTOF-MS/MS and α-amylase, α-glucosidase, tyrosinase, and AchE inhibitory activity were determined. The maximum extraction rate of SPPE was 7.56 mg GAE/g and the polyphenol purity reached 70.5% after macroporous resin and Sephadex LH-20 purification. A total of 50 compounds were identified by UPLC-QTOF-MS/MS. The IC50 values of SPPE were 334.9 μg/mL, 6.290 μg /mL, 0.834 mg /mL and 0.6538 mg /mL for α-amylase, α-glucosidase, tyrosinase and AchE, respectively. Molecular docking technology further revealed the effects of SPPE on the above enzymes. This study provided information on the potential hypoglycemic, whitening and anti-Alzheimer's disease biological activities of SPPE, which had guiding significance for the purification and development of other seaweed polyphenols.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:19 |
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| Enthalten in: |
PloS one - 19(2024), 1 vom: 01., Seite e0297434 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jiang, Haiyun [VerfasserIn] |
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| Links: |
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| Themen: |
Alpha-Amylases |
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| Anmerkungen: |
Date Completed 01.02.2024 Date Revised 01.02.2024 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.1371/journal.pone.0297434 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a This study aimed to obtain a high yield and purity of Sargassum pallidum polyphenol extracts (SPPE) and study its enzyme activity. Fresh Sargassum pallidum seaweed was selected for optimization of ultrasound-assisted extraction (UAE) conditions and purification conditions using macroporous resin and Sephadex LH20 to obtain SPPE. The SPPE was characterized using UPLC-QTOF-MS/MS and α-amylase, α-glucosidase, tyrosinase, and AchE inhibitory activity were determined. The maximum extraction rate of SPPE was 7.56 mg GAE/g and the polyphenol purity reached 70.5% after macroporous resin and Sephadex LH-20 purification. A total of 50 compounds were identified by UPLC-QTOF-MS/MS. The IC50 values of SPPE were 334.9 μg/mL, 6.290 μg /mL, 0.834 mg /mL and 0.6538 mg /mL for α-amylase, α-glucosidase, tyrosinase and AchE, respectively. Molecular docking technology further revealed the effects of SPPE on the above enzymes. This study provided information on the potential hypoglycemic, whitening and anti-Alzheimer's disease biological activities of SPPE, which had guiding significance for the purification and development of other seaweed polyphenols | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Polyphenols |2 NLM | |
| 650 | 7 | |a Monophenol Monooxygenase |2 NLM | |
| 650 | 7 | |a EC 1.14.18.1 |2 NLM | |
| 650 | 7 | |a alpha-Glucosidases |2 NLM | |
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| 650 | 7 | |a alpha-Amylases |2 NLM | |
| 650 | 7 | |a EC 3.2.1.1 |2 NLM | |
| 650 | 7 | |a Antioxidants |2 NLM | |
| 650 | 7 | |a Plant Extracts |2 NLM | |
| 700 | 1 | |a Kong, Li |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Hongguang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Zhenzhen |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Caiping |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jianhui |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Yuxin |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Jinyang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Yue |e verfasserin |4 aut | |
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