Multi-region calcium imaging in freely behaving mice with ultra-compact head-mounted fluorescence microscopes
© The Author(s) 2023. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd..
To investigate the circuit-level neural mechanisms of behavior, simultaneous imaging of neuronal activity in multiple cortical and subcortical regions is highly desired. Miniature head-mounted microscopes offer the capability of calcium imaging in freely behaving animals. However, implanting multiple microscopes on a mouse brain remains challenging due to space constraints and the cumbersome weight of the equipment. Here, we present TINIscope, a Tightly Integrated Neuronal Imaging microscope optimized for electronic and opto-mechanical design. With its compact and lightweight design of 0.43 g, TINIscope enables unprecedented simultaneous imaging of behavior-relevant activity in up to four brain regions in mice. Proof-of-concept experiments with TINIscope recorded over 1000 neurons in four hippocampal subregions and revealed concurrent activity patterns spanning across these regions. Moreover, we explored potential multi-modal experimental designs by integrating additional modules for optogenetics, electrical stimulation or local field potential recordings. Overall, TINIscope represents a timely and indispensable tool for studying the brain-wide interregional coordination that underlies unrestrained behaviors.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
|---|---|
| Enthalten in: |
National science review - 11(2024), 1 vom: 01. Jan., Seite nwad294 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Xue, Feng [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Calcium imaging |
|---|
| Anmerkungen: |
Date Revised 26.04.2024 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.1093/nsr/nwad294 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM367787091 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM367787091 | ||
| 003 | DE-627 | ||
| 005 | 20240426233622.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240130s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1093/nsr/nwad294 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1388.xml |
| 035 | |a (DE-627)NLM367787091 | ||
| 035 | |a (NLM)38288367 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Xue, Feng |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Multi-region calcium imaging in freely behaving mice with ultra-compact head-mounted fluorescence microscopes |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 26.04.2024 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © The Author(s) 2023. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd. | ||
| 520 | |a To investigate the circuit-level neural mechanisms of behavior, simultaneous imaging of neuronal activity in multiple cortical and subcortical regions is highly desired. Miniature head-mounted microscopes offer the capability of calcium imaging in freely behaving animals. However, implanting multiple microscopes on a mouse brain remains challenging due to space constraints and the cumbersome weight of the equipment. Here, we present TINIscope, a Tightly Integrated Neuronal Imaging microscope optimized for electronic and opto-mechanical design. With its compact and lightweight design of 0.43 g, TINIscope enables unprecedented simultaneous imaging of behavior-relevant activity in up to four brain regions in mice. Proof-of-concept experiments with TINIscope recorded over 1000 neurons in four hippocampal subregions and revealed concurrent activity patterns spanning across these regions. Moreover, we explored potential multi-modal experimental designs by integrating additional modules for optogenetics, electrical stimulation or local field potential recordings. Overall, TINIscope represents a timely and indispensable tool for studying the brain-wide interregional coordination that underlies unrestrained behaviors | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a calcium imaging | |
| 650 | 4 | |a hippocampus | |
| 650 | 4 | |a miniature microscope | |
| 650 | 4 | |a multimodal integration | |
| 650 | 4 | |a multiple sites | |
| 700 | 1 | |a Li, Fei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Ke-Ming |e verfasserin |4 aut | |
| 700 | 1 | |a Ding, Lufeng |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Xingtao |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Fang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Danke |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Mingzhai |e verfasserin |4 aut | |
| 700 | 1 | |a Lau, Pak-Ming |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Qingyuan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Pengcheng |e verfasserin |4 aut | |
| 700 | 1 | |a Bi, Guo-Qiang |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t National science review |d 2014 |g 11(2024), 1 vom: 01. Jan., Seite nwad294 |w (DE-627)NLM243751931 |x 2053-714X |7 nnns |
| 773 | 1 | 8 | |g volume:11 |g year:2024 |g number:1 |g day:01 |g month:01 |g pages:nwad294 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1093/nsr/nwad294 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 11 |j 2024 |e 1 |b 01 |c 01 |h nwad294 | ||