Detection of dsRNA with Fluorescence In Situ Hybridization (FISH)
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
Fluorescence in situ hybridization (FISH) is a powerful technique used for detecting and localizing specific nucleic acid sequences in cells or tissues. Double-stranded RNA (dsRNA) is a type of RNA with complementary strands, highly produced during the replication cycle of RNA viruses. dsRNA plays an essential role in many biological processes, including the activation of RNA silencing. Here, we present an overview of how FISH can be employed to detect and locate dsRNA. The detection and localization of dsRNA through FISH provide valuable insights into RNA-mediated processes and their roles in various biological phenomena.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2771 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2771(2024) vom: 29., Seite 35-38 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhou, Wenling [VerfasserIn] |
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| Links: |
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| Themen: |
DsRNA detection |
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| Anmerkungen: |
Date Completed 30.01.2024 Date Revised 30.01.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-3702-9_6 |
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| funding: |
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| 520 | |a Fluorescence in situ hybridization (FISH) is a powerful technique used for detecting and localizing specific nucleic acid sequences in cells or tissues. Double-stranded RNA (dsRNA) is a type of RNA with complementary strands, highly produced during the replication cycle of RNA viruses. dsRNA plays an essential role in many biological processes, including the activation of RNA silencing. Here, we present an overview of how FISH can be employed to detect and locate dsRNA. The detection and localization of dsRNA through FISH provide valuable insights into RNA-mediated processes and their roles in various biological phenomena | ||
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| 700 | 1 | |a Zhang, Xiaoming |e verfasserin |4 aut | |
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