Ribosomal frameshifting at normal codon repeats recodes functional chimeric proteins in human
© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research..
Ribosomal frameshifting refers to the process that ribosomes slip into +1 or -1 reading frame, thus produce chimeric trans-frame proteins. In viruses and bacteria, programmed ribosomal frameshifting can produce essential trans-frame proteins for viral replication or regulation of other biological processes. In humans, however, functional trans-frame protein derived from ribosomal frameshifting is scarcely documented. Combining multiple assays, we show that short codon repeats could act as cis-acting elements that stimulate ribosomal frameshifting in humans, abbreviated as CRFS hereafter. Using proteomic analyses, we identified many putative CRFS events from 32 normal human tissues supported by trans-frame peptides positioned at codon repeats. Finally, we show a CRFS-derived trans-frame protein (HDAC1-FS) functions by antagonizing the activities of HDAC1, thus affecting cell migration and apoptosis. These data suggest a novel type of translational recoding associated with codon repeats, which may expand the coding capacity of mRNA and diversify the regulation in human.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:52 |
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| Enthalten in: |
Nucleic acids research - 52(2024), 5 vom: 21. März, Seite 2463-2479 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ren, Guiping [VerfasserIn] |
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| Anmerkungen: |
Date Completed 22.03.2024 Date Revised 23.03.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/nar/gkae035 |
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| 520 | |a © The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research. | ||
| 520 | |a Ribosomal frameshifting refers to the process that ribosomes slip into +1 or -1 reading frame, thus produce chimeric trans-frame proteins. In viruses and bacteria, programmed ribosomal frameshifting can produce essential trans-frame proteins for viral replication or regulation of other biological processes. In humans, however, functional trans-frame protein derived from ribosomal frameshifting is scarcely documented. Combining multiple assays, we show that short codon repeats could act as cis-acting elements that stimulate ribosomal frameshifting in humans, abbreviated as CRFS hereafter. Using proteomic analyses, we identified many putative CRFS events from 32 normal human tissues supported by trans-frame peptides positioned at codon repeats. Finally, we show a CRFS-derived trans-frame protein (HDAC1-FS) functions by antagonizing the activities of HDAC1, thus affecting cell migration and apoptosis. These data suggest a novel type of translational recoding associated with codon repeats, which may expand the coding capacity of mRNA and diversify the regulation in human | ||
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| 700 | 1 | |a Zhang, Lu |e verfasserin |4 aut | |
| 700 | 1 | |a Gong, Shimin |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Shuang |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Shunkai |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Zhenjing |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiaoyan |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Zhanbiao |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Yingshui |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Longxi |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Jiao |e verfasserin |4 aut | |
| 700 | 1 | |a Lai, Fan |e verfasserin |4 aut | |
| 700 | 1 | |a Dang, Yunkun |e verfasserin |4 aut | |
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