Structural characterization and anti-oxidant activity of polysaccharide HVP-1 from Volvariella volvacea
Copyright © 2024 Elsevier B.V. All rights reserved..
In this study, a novel homogeneous polysaccharide (HVP-1) was purified from the Volvariella volvacea. Its structural characteristics and anti-oxidant activity in vitro were further evaluated. The results revealed that HVP-1 was composed of mannose, glucose, galactose and arabinose in a molar ratio (mol %) of 55.37: 15.74: 25.20: 3.69. Its main chain consisted of →4)-β-D-Galp-(1→, →6)-α-D-Glcp-(1→, →3)-α-D-Glcp-(1→, →4)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→. The branched structure α-L-Araf-(1→, →2)-β-D-Glcp-(1→ and →6)-β-D-Manp-(1→ were connected to →3,6)-β-D-Manp-(1→ through the O-3 position. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed that HVP-1 had porous sheet-like structure with a triple helix conformation. Anti-oxidant activity experiments showed that HVP-1 alleviated H2O2-induced oxidative damage by reducing the accumulation of reactive oxygen species, increasing the activity of related enzymes in cells, and activating the Nrf2/HO-1 signaling pathway. These results suggested that HVP-1 had the potential to be used as a natural anti-oxidant in functional foods and pharmaceuticals.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:261 |
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| Enthalten in: |
International journal of biological macromolecules - 261(2024), Pt 1 vom: 25. März, Seite 129672 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Qilong [VerfasserIn] |
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| Links: |
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| Themen: |
Anti-oxidation activity |
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| Anmerkungen: |
Date Completed 11.03.2024 Date Revised 11.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ijbiomac.2024.129672 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a In this study, a novel homogeneous polysaccharide (HVP-1) was purified from the Volvariella volvacea. Its structural characteristics and anti-oxidant activity in vitro were further evaluated. The results revealed that HVP-1 was composed of mannose, glucose, galactose and arabinose in a molar ratio (mol %) of 55.37: 15.74: 25.20: 3.69. Its main chain consisted of →4)-β-D-Galp-(1→, →6)-α-D-Glcp-(1→, →3)-α-D-Glcp-(1→, →4)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→. The branched structure α-L-Araf-(1→, →2)-β-D-Glcp-(1→ and →6)-β-D-Manp-(1→ were connected to →3,6)-β-D-Manp-(1→ through the O-3 position. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed that HVP-1 had porous sheet-like structure with a triple helix conformation. Anti-oxidant activity experiments showed that HVP-1 alleviated H2O2-induced oxidative damage by reducing the accumulation of reactive oxygen species, increasing the activity of related enzymes in cells, and activating the Nrf2/HO-1 signaling pathway. These results suggested that HVP-1 had the potential to be used as a natural anti-oxidant in functional foods and pharmaceuticals | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Anti-oxidation activity | |
| 650 | 4 | |a Physicochemical properties | |
| 650 | 4 | |a Polysaccharide | |
| 650 | 4 | |a Structural characterization | |
| 650 | 4 | |a Volvariella volvacea | |
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| 650 | 7 | |a Polysaccharides |2 NLM | |
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| 700 | 1 | |a Li, Mengxin |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Li |e verfasserin |4 aut | |
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