Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
Autoantibodies that recognize self-antigens are believed to have a close relationship with diseases such as autoimmune diseases, cancer, and lifestyle diseases. Analysis of autoantibodies is essential for investigating pathology mechanisms, diagnosis, and therapeutics of these diseases. We developed an autoantibody profiling assay using a cell-free synthesized protein array and high-throughput screening technology. Our assay system can sensitively detect interaction between recombinant antigen protein and autoantibody and efficiently analyze autoantibody profiling in patients' sera.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2766 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2766(2024) vom: 25., Seite 107-128 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Takeda, Hiroyuki [VerfasserIn] |
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| Links: |
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| Themen: |
AlphaScreen |
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| Anmerkungen: |
Date Completed 26.01.2024 Date Revised 26.01.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-3682-4_12 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Autoantibodies that recognize self-antigens are believed to have a close relationship with diseases such as autoimmune diseases, cancer, and lifestyle diseases. Analysis of autoantibodies is essential for investigating pathology mechanisms, diagnosis, and therapeutics of these diseases. We developed an autoantibody profiling assay using a cell-free synthesized protein array and high-throughput screening technology. Our assay system can sensitively detect interaction between recombinant antigen protein and autoantibody and efficiently analyze autoantibody profiling in patients' sera | ||
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