Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes
© 2024. The Author(s)..
Inorganic pyrophosphate is a key molecule in many biological processes from DNA synthesis to cell metabolism. Here we introduce sp3-functionalized (6,5) single-walled carbon nanotubes (SWNTs) with red-shifted defect emission as near-infrared luminescent probes for the optical detection and quantification of inorganic pyrophosphate. The sensing scheme is based on the immobilization of Cu2+ ions on the SWNT surface promoted by coordination to covalently attached aryl alkyne groups and a triazole complex. The presence of Cu2+ ions on the SWNT surface causes fluorescence quenching via photoinduced electron transfer, which is reversed by copper-complexing analytes such as pyrophosphate. The differences in the fluorescence response of sp3-defect to pristine nanotube emission enables reproducible ratiometric measurements in a wide concentration window. Biocompatible, phospholipid-polyethylene glycol-coated SWNTs with such sp3 defects are employed for the detection of pyrophosphate in cell lysate and for monitoring the progress of DNA synthesis in a polymerase chain reaction. This robust ratiometric and near-infrared luminescent probe for pyrophosphate may serve as a starting point for the rational design of nanotube-based biosensors.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Nature communications - 15(2024), 1 vom: 24. Jan., Seite 706 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Settele, Simon [VerfasserIn] |
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| Links: |
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| Themen: |
4E862E7GRQ |
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| Anmerkungen: |
Date Completed 26.01.2024 Date Revised 27.01.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41467-024-45052-1 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Inorganic pyrophosphate is a key molecule in many biological processes from DNA synthesis to cell metabolism. Here we introduce sp3-functionalized (6,5) single-walled carbon nanotubes (SWNTs) with red-shifted defect emission as near-infrared luminescent probes for the optical detection and quantification of inorganic pyrophosphate. The sensing scheme is based on the immobilization of Cu2+ ions on the SWNT surface promoted by coordination to covalently attached aryl alkyne groups and a triazole complex. The presence of Cu2+ ions on the SWNT surface causes fluorescence quenching via photoinduced electron transfer, which is reversed by copper-complexing analytes such as pyrophosphate. The differences in the fluorescence response of sp3-defect to pristine nanotube emission enables reproducible ratiometric measurements in a wide concentration window. Biocompatible, phospholipid-polyethylene glycol-coated SWNTs with such sp3 defects are employed for the detection of pyrophosphate in cell lysate and for monitoring the progress of DNA synthesis in a polymerase chain reaction. This robust ratiometric and near-infrared luminescent probe for pyrophosphate may serve as a starting point for the rational design of nanotube-based biosensors | ||
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| 700 | 1 | |a Jung, Sebastian |e verfasserin |4 aut | |
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| 700 | 1 | |a Li, Han |e verfasserin |4 aut | |
| 700 | 1 | |a Flavel, Benjamin S |e verfasserin |4 aut | |
| 700 | 1 | |a Hashmi, A Stephen K |e verfasserin |4 aut | |
| 700 | 1 | |a Kruss, Sebastian |e verfasserin |4 aut | |
| 700 | 1 | |a Zaumseil, Jana |e verfasserin |4 aut | |
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