Detection of Plasmodium spp. in asymptomatic blood donors by the new Brazilian NAT PLUS HIV/HBV/HCV/Malaria Bio-Manguinhos kit
© 2024 AABB..
BACKGROUND: Transfusion-transmitted malaria (TTM) is a public health problem in endemic and nonendemic areas. The Brazilian Ministry of Health (MH) requested the development of a nucleic acid amplification test (NAT) for the detection of Plasmodium spp. in public blood centers to increase blood safety.
STUDY DESIGN AND METHODS: The new Brazilian NAT kit named NAT PLUS HIV/HBV/HCV/Malaria Bio-Manguinhos was first implemented in HEMORIO, a public blood center in the city of Rio de Janeiro. Since October 1, 2022, this blood center has been testing all its blood donations for malaria in a pool of six plasma samples to detect Plasmodium spp. by real-time polymerase chain reaction (PCR).
RESULTS: Since the implementation of the NAT PLUS platform until February 2023, HEMORIO has successfully received and tested 200,277 donations. The platform detected two asymptomatic donors in the city of Rio de Janeiro, which is a nonendemic region for malaria. Our analyses suggested a malaria from the Amazon region caused by Plasmodium vivax, in the first case, while an autochthonous transmission case by Plasmodium malariae was identified in the rural area of Rio de Janeiro state.
DISCUSSION: The NAT PLUS platform detects Plasmodium spp. in plasma samples with sensitivity capable of detecting subpatent infections. This is the first time worldwide that a group developed and implemented molecular diagnosis for Plasmodium spp. to be used by public blood centers to avoid TTM.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2024 |
---|---|
Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:64 |
---|---|
Enthalten in: |
Transfusion - 64(2024), 3 vom: 01. März, Seite 501-509 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Costa, Elaine [VerfasserIn] |
---|
Links: |
---|
Themen: |
Blood safety |
---|
Anmerkungen: |
Date Completed 18.03.2024 Date Revised 18.03.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1111/trf.17726 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM367493675 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM367493675 | ||
003 | DE-627 | ||
005 | 20240318234119.0 | ||
007 | cr uuu---uuuuu | ||
008 | 240123s2024 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1111/trf.17726 |2 doi | |
028 | 5 | 2 | |a pubmed24n1334.xml |
035 | |a (DE-627)NLM367493675 | ||
035 | |a (NLM)38258881 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Costa, Elaine |e verfasserin |4 aut | |
245 | 1 | 0 | |a Detection of Plasmodium spp. in asymptomatic blood donors by the new Brazilian NAT PLUS HIV/HBV/HCV/Malaria Bio-Manguinhos kit |
264 | 1 | |c 2024 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 18.03.2024 | ||
500 | |a Date Revised 18.03.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a © 2024 AABB. | ||
520 | |a BACKGROUND: Transfusion-transmitted malaria (TTM) is a public health problem in endemic and nonendemic areas. The Brazilian Ministry of Health (MH) requested the development of a nucleic acid amplification test (NAT) for the detection of Plasmodium spp. in public blood centers to increase blood safety | ||
520 | |a STUDY DESIGN AND METHODS: The new Brazilian NAT kit named NAT PLUS HIV/HBV/HCV/Malaria Bio-Manguinhos was first implemented in HEMORIO, a public blood center in the city of Rio de Janeiro. Since October 1, 2022, this blood center has been testing all its blood donations for malaria in a pool of six plasma samples to detect Plasmodium spp. by real-time polymerase chain reaction (PCR) | ||
520 | |a RESULTS: Since the implementation of the NAT PLUS platform until February 2023, HEMORIO has successfully received and tested 200,277 donations. The platform detected two asymptomatic donors in the city of Rio de Janeiro, which is a nonendemic region for malaria. Our analyses suggested a malaria from the Amazon region caused by Plasmodium vivax, in the first case, while an autochthonous transmission case by Plasmodium malariae was identified in the rural area of Rio de Janeiro state | ||
520 | |a DISCUSSION: The NAT PLUS platform detects Plasmodium spp. in plasma samples with sensitivity capable of detecting subpatent infections. This is the first time worldwide that a group developed and implemented molecular diagnosis for Plasmodium spp. to be used by public blood centers to avoid TTM | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a blood safety | |
650 | 4 | |a malaria | |
650 | 4 | |a nucleic acid amplification tests | |
650 | 4 | |a transfusion-transmitted malaria | |
700 | 1 | |a Rocha, Daniele |e verfasserin |4 aut | |
700 | 1 | |a Lopes, Josiane Iole França |e verfasserin |4 aut | |
700 | 1 | |a Andrade, Elisabete |e verfasserin |4 aut | |
700 | 1 | |a Cardoso, Pedro |e verfasserin |4 aut | |
700 | 1 | |a Ribeiro, Marisa |e verfasserin |4 aut | |
700 | 1 | |a Fontana-Maurell, Marcela |e verfasserin |4 aut | |
700 | 1 | |a Vicentino, Amanda Roberta Revoredo |e verfasserin |4 aut | |
700 | 1 | |a Calazans, Alexandre Rodrigues |e verfasserin |4 aut | |
700 | 1 | |a Arruda, Monica Barcellos |e verfasserin |4 aut | |
700 | 1 | |a Mesquita, Camila de Amorim |e verfasserin |4 aut | |
700 | 1 | |a Ferreira, Antonio Gomes Pinto |e verfasserin |4 aut | |
700 | 1 | |a Amorim Filho, Luiz |e verfasserin |4 aut | |
700 | 1 | |a Alvarez, Patrícia |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Transfusion |d 1961 |g 64(2024), 3 vom: 01. März, Seite 501-509 |w (DE-627)NLM00001107X |x 1537-2995 |7 nnns |
773 | 1 | 8 | |g volume:64 |g year:2024 |g number:3 |g day:01 |g month:03 |g pages:501-509 |
856 | 4 | 0 | |u http://dx.doi.org/10.1111/trf.17726 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 64 |j 2024 |e 3 |b 01 |c 03 |h 501-509 |