Platelet-rich plasma-derived exosomes boost mesenchymal stem cells to promote peripheral nerve regeneration
Copyright © 2024 Elsevier B.V. All rights reserved..
Peripheral nerve injury (PNI) remains a severe clinical problem with debilitating consequences. Mesenchymal stem cell (MSC)-based therapy is promising, but the problems of poor engraftment and insufficient neurotrophic effects need to be overcome. Herein, we isolated platelet-rich plasma-derived exosomes (PRP-Exos), which contain abundant bioactive molecules, and investigated their potential to increase the regenerative capacity of MSCs. We observed that PRP-Exos significantly increased MSC proliferation, viability, and mobility, decreased MSC apoptosis under stress, maintained MSC stemness, and attenuated MSC senescence. In vivo, PRP-Exo-treated MSCs (pExo-MSCs) exhibited an increased retention rate and heightened therapeutic efficacy, as indicated by increased axonal regeneration, remyelination, and recovery of neurological function in a PNI model. In vitro, pExo-MSCs coculture promoted Schwann cell proliferation and dorsal root ganglion axon growth. Moreover, the increased neurotrophic behaviour of pExo-MSCs was mediated by trophic factors, particularly glia-derived neurotrophic factor (GDNF), and PRP-Exos activated the PI3K/Akt signalling pathway in MSCs, leading to the observed phenotypes. These findings demonstrate that PRP-Exos may be novel agents for increasing the ability of MSCs to promote neural repair and regeneration in patients with PNI.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:367 |
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| Enthalten in: |
Journal of controlled release : official journal of the Controlled Release Society - 367(2024) vom: 31. März, Seite 265-282 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Yongyi [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 25.03.2024 Date Revised 25.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jconrel.2024.01.043 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2024 Elsevier B.V. All rights reserved. | ||
| 520 | |a Peripheral nerve injury (PNI) remains a severe clinical problem with debilitating consequences. Mesenchymal stem cell (MSC)-based therapy is promising, but the problems of poor engraftment and insufficient neurotrophic effects need to be overcome. Herein, we isolated platelet-rich plasma-derived exosomes (PRP-Exos), which contain abundant bioactive molecules, and investigated their potential to increase the regenerative capacity of MSCs. We observed that PRP-Exos significantly increased MSC proliferation, viability, and mobility, decreased MSC apoptosis under stress, maintained MSC stemness, and attenuated MSC senescence. In vivo, PRP-Exo-treated MSCs (pExo-MSCs) exhibited an increased retention rate and heightened therapeutic efficacy, as indicated by increased axonal regeneration, remyelination, and recovery of neurological function in a PNI model. In vitro, pExo-MSCs coculture promoted Schwann cell proliferation and dorsal root ganglion axon growth. Moreover, the increased neurotrophic behaviour of pExo-MSCs was mediated by trophic factors, particularly glia-derived neurotrophic factor (GDNF), and PRP-Exos activated the PI3K/Akt signalling pathway in MSCs, leading to the observed phenotypes. These findings demonstrate that PRP-Exos may be novel agents for increasing the ability of MSCs to promote neural repair and regeneration in patients with PNI | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cell therapy | |
| 650 | 4 | |a Glia-derived neurotrophic factor | |
| 650 | 4 | |a Nerve regeneration | |
| 650 | 4 | |a Peripheral nerve injury | |
| 650 | 4 | |a Platelet-rich plasma-derived exosomes | |
| 650 | 4 | |a Umbilical cord-derived mesenchymal stem cell | |
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| 700 | 1 | |a Yi, Dan |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Quan |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Jiangbei |e verfasserin |4 aut | |
| 700 | 1 | |a Geng, Xiaodong |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jinwei |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Chuang |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Mengyu |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Chao |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Shuaixuan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Zhen |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Molin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Yaqiong |e verfasserin |4 aut | |
| 700 | 1 | |a Peng, Nan |e verfasserin |4 aut | |
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