Exploration of reference genes for the development of a diagnostic kit on vascular aging in human saliva
Identifying reliable biomarkers in saliva can be a promising approach to developing a rapid diagnostic kit for detecting vascular aging. This study investigated the most suitable reference gene for polymerase chain reaction (PCR) in saliva that is not affected by vascular aging variables. Whole saliva samples were collected to assess the expression of reference genes: actin beta (ACTB), 18S ribosomal RNA (18S rRNA), beta-2-microglobulin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The most abundantly expressed gene was 18S rRNA, and the least expressed gene was GAPDH. Four genes were ranked according to their relative stability, as determined by mathematical algorithms, indicating that ACTB and 18S rRNA were stably expressed as reference genes. 18S rRNA was identified as the most promising reference gene for detecting systemic diseases using saliva from patients with vascular aging in these limited experimental conditions.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:43 |
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| Enthalten in: |
Dental materials journal - 43(2024), 2 vom: 29. März, Seite 172-178 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Miyoshi, Shinsuke [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 02.04.2024 Date Revised 02.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.4012/dmj.2023-242 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM367371251 |
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| 520 | |a Identifying reliable biomarkers in saliva can be a promising approach to developing a rapid diagnostic kit for detecting vascular aging. This study investigated the most suitable reference gene for polymerase chain reaction (PCR) in saliva that is not affected by vascular aging variables. Whole saliva samples were collected to assess the expression of reference genes: actin beta (ACTB), 18S ribosomal RNA (18S rRNA), beta-2-microglobulin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The most abundantly expressed gene was 18S rRNA, and the least expressed gene was GAPDH. Four genes were ranked according to their relative stability, as determined by mathematical algorithms, indicating that ACTB and 18S rRNA were stably expressed as reference genes. 18S rRNA was identified as the most promising reference gene for detecting systemic diseases using saliva from patients with vascular aging in these limited experimental conditions | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Human whole saliva | |
| 650 | 4 | |a Reference gene | |
| 650 | 4 | |a Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) | |
| 650 | 4 | |a Vascular aging | |
| 650 | 7 | |a RNA, Ribosomal, 18S |2 NLM | |
| 650 | 7 | |a Glyceraldehyde-3-Phosphate Dehydrogenases |2 NLM | |
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| 700 | 1 | |a Kawamoto, Akiyo |e verfasserin |4 aut | |
| 700 | 1 | |a Ninomiya, Yuichi |e verfasserin |4 aut | |
| 700 | 1 | |a Hamada, Yoshihiro |e verfasserin |4 aut | |
| 700 | 1 | |a Shimizu, Hideo |e verfasserin |4 aut | |
| 700 | 1 | |a Honda, Yoshitomo |e verfasserin |4 aut | |
| 700 | 1 | |a Takahashi, Kazuya |e verfasserin |4 aut | |
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