Identification of dysregulation of sphingolipids in retinoblastoma using liquid chromatography-mass spectrometry
Copyright © 2024 Elsevier Ltd. All rights reserved..
Retinoblastoma (RB) is a rare ocular cancer seen in children that counts for approximately 3% of all childhood cancers. It is found that mutation in RB1, a tumour Suppressor Gene on chromosome 13 as the cause of malignancy. Retinoblastoma protein is the target for ceramide to cause apoptosis. We studied lipidomics of two RB cell lines, one aggressive cell line (NCC-RbC-51) derived from a metastatic site and one non aggressive cell line (WERI-Rb1) in comparison with a control cell line (MIO-M1). Lipid profiles of all the cell lines were studied using high resolution mass spectrometer coupled to high performance liquid chromatography. Data acquired from all the three cell lines in positive mode were analyzed to identify differentially expressed metabolites. Several phospholipids and lysophospholipids were found to be dysregulated. We observed upregulation of hexosyl ceramides, and down regulation of dihydroceramides and higher order sphingoglycolipids hinting at a hindered sphingolipid biosynthesis. The results obtained from liquid chromatography-mass spectrometry are validated by using qPCR and it was observed that genes involved in ceramide biosynthesis pathway are getting down regulated.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:240 |
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| Enthalten in: |
Experimental eye research - 240(2024) vom: 31. März, Seite 109798 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Khade, Omkar Surendra [VerfasserIn] |
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| Links: |
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| Themen: |
Ceramides |
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| Anmerkungen: |
Date Completed 05.03.2024 Date Revised 05.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.exer.2024.109798 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Retinoblastoma (RB) is a rare ocular cancer seen in children that counts for approximately 3% of all childhood cancers. It is found that mutation in RB1, a tumour Suppressor Gene on chromosome 13 as the cause of malignancy. Retinoblastoma protein is the target for ceramide to cause apoptosis. We studied lipidomics of two RB cell lines, one aggressive cell line (NCC-RbC-51) derived from a metastatic site and one non aggressive cell line (WERI-Rb1) in comparison with a control cell line (MIO-M1). Lipid profiles of all the cell lines were studied using high resolution mass spectrometer coupled to high performance liquid chromatography. Data acquired from all the three cell lines in positive mode were analyzed to identify differentially expressed metabolites. Several phospholipids and lysophospholipids were found to be dysregulated. We observed upregulation of hexosyl ceramides, and down regulation of dihydroceramides and higher order sphingoglycolipids hinting at a hindered sphingolipid biosynthesis. The results obtained from liquid chromatography-mass spectrometry are validated by using qPCR and it was observed that genes involved in ceramide biosynthesis pathway are getting down regulated | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Drug resistance | |
| 650 | 4 | |a Glucosyl ceramide | |
| 650 | 4 | |a Lipidomics | |
| 650 | 4 | |a Mass spectrometry | |
| 650 | 4 | |a Retinoblastoma | |
| 650 | 4 | |a Sphingolipids | |
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| 700 | 1 | |a Chatterjee, Amit |e verfasserin |4 aut | |
| 700 | 1 | |a Gopal, Divya |e verfasserin |4 aut | |
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| 700 | 1 | |a Janakiraman, Narayanan |e verfasserin |4 aut | |
| 700 | 1 | |a Elchuri, Sailaja V |e verfasserin |4 aut | |
| 700 | 1 | |a Gundimeda, Seetaramanjaneyulu |e verfasserin |4 aut | |
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