Early life adversity accelerates hypothalamic drive of pubertal timing in female rats with associated enhanced acoustic startle
Copyright © 2024 Elsevier Inc. All rights reserved..
Early life adversity in the form of childhood maltreatment in humans or as modeled by maternal separation (MS) in rodents is often associated with an earlier emergence of puberty in females. Earlier pubertal initiation is an example of accelerated biological aging and predicts later risk for anxiety in women, especially in populations exposed to early life trauma. Here we investigated external pubertal markers as well as hypothalamic gene expression of pubertal regulators kisspeptin and gonadotropin-releasing hormone, to determine a biological substrate for MS-induced accelerated puberty. We further investigated a mechanism by which developmental stress might regulate pubertal timing. As kisspeptin and gonadotropin-releasing hormone secretion are typically inhibited by corticotropin releasing hormone at its receptor CRH-R1, we hypothesized that MS induces a downregulation of Crhr1 gene transcription in a cell-specific manner. Finally, we explored the association between pubertal timing and anxiety-like behavior in an acoustic startle paradigm, to drive future preclinical research linking accelerated puberty and anxiety. We replicated previous findings that MS leads to earlier puberty in females but not males, and found expression of kisspeptin and gonadotropin-releasing hormone mRNA to be prematurely increased in MS females. RNAscope confirmed increased expression of these genes, and further revealed that kisspeptin-expressing neurons in females were less likely to express Crhr1 after MS. Early puberty was associated with higher acoustic startle magnitude in females. Taken together, these findings indicate precocial maturation of central pubertal timing mechanisms after MS, as well as a potential role of CRH-R1 in these effects and an association with a translational measure of anxiety.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:159 |
|---|---|
| Enthalten in: |
Hormones and behavior - 159(2024) vom: 31. März, Seite 105478 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Granata, Lauren [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
33515-09-2 |
|---|
| Anmerkungen: |
Date Completed 26.02.2024 Date Revised 12.03.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.yhbeh.2024.105478 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM367324512 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM367324512 | ||
| 003 | DE-627 | ||
| 005 | 20240312233622.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240120s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.yhbeh.2024.105478 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1324.xml |
| 035 | |a (DE-627)NLM367324512 | ||
| 035 | |a (NLM)38241961 | ||
| 035 | |a (PII)S0018-506X(24)00003-5 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Granata, Lauren |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Early life adversity accelerates hypothalamic drive of pubertal timing in female rats with associated enhanced acoustic startle |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 26.02.2024 | ||
| 500 | |a Date Revised 12.03.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024 Elsevier Inc. All rights reserved. | ||
| 520 | |a Early life adversity in the form of childhood maltreatment in humans or as modeled by maternal separation (MS) in rodents is often associated with an earlier emergence of puberty in females. Earlier pubertal initiation is an example of accelerated biological aging and predicts later risk for anxiety in women, especially in populations exposed to early life trauma. Here we investigated external pubertal markers as well as hypothalamic gene expression of pubertal regulators kisspeptin and gonadotropin-releasing hormone, to determine a biological substrate for MS-induced accelerated puberty. We further investigated a mechanism by which developmental stress might regulate pubertal timing. As kisspeptin and gonadotropin-releasing hormone secretion are typically inhibited by corticotropin releasing hormone at its receptor CRH-R1, we hypothesized that MS induces a downregulation of Crhr1 gene transcription in a cell-specific manner. Finally, we explored the association between pubertal timing and anxiety-like behavior in an acoustic startle paradigm, to drive future preclinical research linking accelerated puberty and anxiety. We replicated previous findings that MS leads to earlier puberty in females but not males, and found expression of kisspeptin and gonadotropin-releasing hormone mRNA to be prematurely increased in MS females. RNAscope confirmed increased expression of these genes, and further revealed that kisspeptin-expressing neurons in females were less likely to express Crhr1 after MS. Early puberty was associated with higher acoustic startle magnitude in females. Taken together, these findings indicate precocial maturation of central pubertal timing mechanisms after MS, as well as a potential role of CRH-R1 in these effects and an association with a translational measure of anxiety | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Acoustic startle | |
| 650 | 4 | |a Adolescence | |
| 650 | 4 | |a Maternal separation | |
| 650 | 4 | |a Puberty | |
| 650 | 7 | |a Kisspeptins |2 NLM | |
| 650 | 7 | |a Gonadotropin-Releasing Hormone |2 NLM | |
| 650 | 7 | |a 33515-09-2 |2 NLM | |
| 700 | 1 | |a Fanikos, Michaela |e verfasserin |4 aut | |
| 700 | 1 | |a Brenhouse, Heather C |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Hormones and behavior |d 1972 |g 159(2024) vom: 31. März, Seite 105478 |w (DE-627)NLM000202312 |x 1095-6867 |7 nnns |
| 773 | 1 | 8 | |g volume:159 |g year:2024 |g day:31 |g month:03 |g pages:105478 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.yhbeh.2024.105478 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 159 |j 2024 |b 31 |c 03 |h 105478 | ||