Structural insights into RNA cleavage by a novel family of bacterial RNases
Processing of RNA is a key regulatory mechanism for all living systems. We recently discovered a novel family of endoribonucleases that is conserved across all bacteria. Here, using crystallography, cryo-EM microscopy, biochemical, biophysical, and mass spectrometry techniques, we are able to shed light on a novel RNA cleavage mechanism in bacteria. We show that YicC, the prototypical member of this family, forms a hexameric channel that closes down on a 26-mer RNA substrate, and find that it cleaves across an RNA hairpin to generate several short fragments.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2023 |
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| Enthalten in: |
Research square - (2023) vom: 27. Dez. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wu, Ruoxi [VerfasserIn] |
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| Themen: |
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| Anmerkungen: |
Date Revised 10.02.2024 published: Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.21203/rs.3.rs-3788707/v1 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM367253291 |
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| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Processing of RNA is a key regulatory mechanism for all living systems. We recently discovered a novel family of endoribonucleases that is conserved across all bacteria. Here, using crystallography, cryo-EM microscopy, biochemical, biophysical, and mass spectrometry techniques, we are able to shed light on a novel RNA cleavage mechanism in bacteria. We show that YicC, the prototypical member of this family, forms a hexameric channel that closes down on a 26-mer RNA substrate, and find that it cleaves across an RNA hairpin to generate several short fragments | ||
| 650 | 4 | |a Preprint | |
| 700 | 1 | |a Barnes, Sarah |e verfasserin |4 aut | |
| 700 | 1 | |a Dahlin, Heather |e verfasserin |4 aut | |
| 700 | 1 | |a Khamrui, Susmita |e verfasserin |4 aut | |
| 700 | 1 | |a Ingle, Shakti |e verfasserin |4 aut | |
| 700 | 1 | |a Xiang, Yufei |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Bechhofer, David |e verfasserin |4 aut | |
| 700 | 1 | |a Lazarus, Michael |e verfasserin |4 aut | |
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