Insights on Protective Effect of Platelet Rich Plasma and Tadalafil on Testicular Ischemia/Reperfusion Injury in Rats Exposed to Testicular Torsion/Detorsion
© Copyright by the Author(s). Published by Cell Physiol Biochem Press..
BACKGROUND/AIMS: Ischemic reperfusion (I-R) injury is greatly influenced by the testicular torsion/detorsion process (TDP). In this instance, the anti-inflammatory properties of plateletrich plasma (PRP) combined with tadalafil (Td) significantly promote tissue healing in the I-R injury model.
METHODS: Five groups of rats were created: the control group, the I-R group not receiving any therapy, the I-R group receiving a single dosage of Td (0.25 mg/kg, I.P.), the I-R group receiving a single dose of PRP (80 l, intratesticular), and the I-R group receiving both Td and PRP. Sperm morphology, motility, and histology were assessed. The levels of TNF-, BAX, antioxidant status, and testosterone were measured. Additionally, E-selectin expression was done.
RESULTS: PRP reduced oxidative stress, inflammation, and apoptosis while also boosting testosterone levels, which alleviated I-R injury. Otherwise, PRP reduces E-selectin expression, which modifies the pathways that control endothelial function. Td also partially demonstrated its testicular-protective activity at the same time.
CONCLUSION: PRP's proven anti-inflammatory, antioxidant, and antiapoptotic potentials make it a natural treatment for testicular harm caused by tadalafil. For the first time, it was demonstrated that PRP therapy restored the functionality of the vascular endothelium, specifically the control of E-selectin expression. Combining Td and PRP therapy may be a promising strategy for improving response to PDE5 inhibitors.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:58 |
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| Enthalten in: |
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology - 58(2024), 1 vom: 16. Jan., Seite 14-32 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Abdel Ghaffar, Dalia M [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 19.01.2024 Date Revised 19.01.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.33594/000000680 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM367227657 |
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| 100 | 1 | |a Abdel Ghaffar, Dalia M |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Insights on Protective Effect of Platelet Rich Plasma and Tadalafil on Testicular Ischemia/Reperfusion Injury in Rats Exposed to Testicular Torsion/Detorsion |
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| 500 | |a Date Revised 19.01.2024 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © Copyright by the Author(s). Published by Cell Physiol Biochem Press. | ||
| 520 | |a BACKGROUND/AIMS: Ischemic reperfusion (I-R) injury is greatly influenced by the testicular torsion/detorsion process (TDP). In this instance, the anti-inflammatory properties of plateletrich plasma (PRP) combined with tadalafil (Td) significantly promote tissue healing in the I-R injury model | ||
| 520 | |a METHODS: Five groups of rats were created: the control group, the I-R group not receiving any therapy, the I-R group receiving a single dosage of Td (0.25 mg/kg, I.P.), the I-R group receiving a single dose of PRP (80 l, intratesticular), and the I-R group receiving both Td and PRP. Sperm morphology, motility, and histology were assessed. The levels of TNF-, BAX, antioxidant status, and testosterone were measured. Additionally, E-selectin expression was done | ||
| 520 | |a RESULTS: PRP reduced oxidative stress, inflammation, and apoptosis while also boosting testosterone levels, which alleviated I-R injury. Otherwise, PRP reduces E-selectin expression, which modifies the pathways that control endothelial function. Td also partially demonstrated its testicular-protective activity at the same time | ||
| 520 | |a CONCLUSION: PRP's proven anti-inflammatory, antioxidant, and antiapoptotic potentials make it a natural treatment for testicular harm caused by tadalafil. For the first time, it was demonstrated that PRP therapy restored the functionality of the vascular endothelium, specifically the control of E-selectin expression. Combining Td and PRP therapy may be a promising strategy for improving response to PDE5 inhibitors | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a E-selectin; Ischemia reperfusion injury; Platelet rich plasma; Tadalafil; Testicular torsion/detorsion | |
| 650 | 7 | |a Tadalafil |2 NLM | |
| 650 | 7 | |a 742SXX0ICT |2 NLM | |
| 650 | 7 | |a E-Selectin |2 NLM | |
| 650 | 7 | |a Antioxidants |2 NLM | |
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| 650 | 7 | |a Anti-Inflammatory Agents |2 NLM | |
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| 700 | 1 | |a Eldken, Zienab Helmy |e verfasserin |4 aut | |
| 700 | 1 | |a Sultan, Mohammed S |e verfasserin |4 aut | |
| 700 | 1 | |a Khalil, Rania M |e verfasserin |4 aut | |
| 700 | 1 | |a Sakr, Noha Hamma |e verfasserin |4 aut | |
| 700 | 1 | |a Eissa, Hanan |e verfasserin |4 aut | |
| 700 | 1 | |a Safwat, Sally M |e verfasserin |4 aut | |
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