Tissue-resident macrophages exacerbate lung injury after remote sterile damage
© 2024. The Author(s), under exclusive licence to CSI and USTC..
Remote organ injury, which is a common secondary complication of sterile tissue damage, is a major cause of poor prognosis and is difficult to manage. Here, we report the critical role of tissue-resident macrophages in lung injury after trauma or stroke through the inflammatory response. We found that depleting tissue-resident macrophages rather than disrupting the recruitment of monocyte-derived macrophages attenuated lung injury after trauma or stroke. Our findings revealed that the release of circulating alarmins from sites of distant sterile tissue damage triggered an inflammatory response in lung-resident macrophages by binding to receptor for advanced glycation end products (RAGE) on the membrane, which activated epidermal growth factor receptor (EGFR). Mechanistically, ligand-activated RAGE triggered EGFR activation through an interaction, leading to Rab5-mediated RAGE internalization and EGFR phosphorylation, which subsequently recruited and activated P38; this, in turn, promoted RAGE translation and trafficking to the plasma membrane to increase the cellular response to RAGE ligands, consequently exacerbating inflammation. Our study also showed that the loss of RAGE or EGFR expression by adoptive transfer of macrophages, blocking the function of RAGE with a neutralizing antibody, or pharmacological inhibition of EGFR activation in macrophages could protect against trauma- or stroke-induced remote lung injury. Therefore, our study revealed that targeting the RAGE-EGFR signaling pathway in tissue-resident macrophages is a potential therapeutic approach for treating secondary complications of sterile damage.
| Errataetall: | |
|---|---|
| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
|---|---|
| Enthalten in: |
Cellular & molecular immunology - 21(2024), 4 vom: 10. Apr., Seite 332-348 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Zhong, Hanhui [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
EC 2.7.10.1 |
|---|
| Anmerkungen: |
Date Completed 01.04.2024 Date Revised 10.04.2024 published: Print-Electronic ErratumIn: Cell Mol Immunol. 2024 Mar 1;:. - PMID 38429370 Citation Status MEDLINE |
|---|
| doi: |
10.1038/s41423-024-01125-1 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM367192993 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM367192993 | ||
| 003 | DE-627 | ||
| 005 | 20240410232415.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240117s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s41423-024-01125-1 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1371.xml |
| 035 | |a (DE-627)NLM367192993 | ||
| 035 | |a (NLM)38228746 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zhong, Hanhui |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Tissue-resident macrophages exacerbate lung injury after remote sterile damage |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 01.04.2024 | ||
| 500 | |a Date Revised 10.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a ErratumIn: Cell Mol Immunol. 2024 Mar 1;:. - PMID 38429370 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2024. The Author(s), under exclusive licence to CSI and USTC. | ||
| 520 | |a Remote organ injury, which is a common secondary complication of sterile tissue damage, is a major cause of poor prognosis and is difficult to manage. Here, we report the critical role of tissue-resident macrophages in lung injury after trauma or stroke through the inflammatory response. We found that depleting tissue-resident macrophages rather than disrupting the recruitment of monocyte-derived macrophages attenuated lung injury after trauma or stroke. Our findings revealed that the release of circulating alarmins from sites of distant sterile tissue damage triggered an inflammatory response in lung-resident macrophages by binding to receptor for advanced glycation end products (RAGE) on the membrane, which activated epidermal growth factor receptor (EGFR). Mechanistically, ligand-activated RAGE triggered EGFR activation through an interaction, leading to Rab5-mediated RAGE internalization and EGFR phosphorylation, which subsequently recruited and activated P38; this, in turn, promoted RAGE translation and trafficking to the plasma membrane to increase the cellular response to RAGE ligands, consequently exacerbating inflammation. Our study also showed that the loss of RAGE or EGFR expression by adoptive transfer of macrophages, blocking the function of RAGE with a neutralizing antibody, or pharmacological inhibition of EGFR activation in macrophages could protect against trauma- or stroke-induced remote lung injury. Therefore, our study revealed that targeting the RAGE-EGFR signaling pathway in tissue-resident macrophages is a potential therapeutic approach for treating secondary complications of sterile damage | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a RAGE | |
| 650 | 4 | |a remote sterile damage | |
| 650 | 4 | |a tissue-resident macrophage | |
| 650 | 7 | |a Receptor for Advanced Glycation End Products |2 NLM | |
| 650 | 7 | |a ErbB Receptors |2 NLM | |
| 650 | 7 | |a EC 2.7.10.1 |2 NLM | |
| 700 | 1 | |a Ji, Jingjing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhuang, Jinling |e verfasserin |4 aut | |
| 700 | 1 | |a Xiong, Ziying |e verfasserin |4 aut | |
| 700 | 1 | |a Xie, Pengyun |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiaolei |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Jundi |e verfasserin |4 aut | |
| 700 | 1 | |a Tian, Wangli |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Xiaoyang |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Jing |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cellular & molecular immunology |d 2004 |g 21(2024), 4 vom: 10. Apr., Seite 332-348 |w (DE-627)NLM158038010 |x 2042-0226 |7 nnns |
| 773 | 1 | 8 | |g volume:21 |g year:2024 |g number:4 |g day:10 |g month:04 |g pages:332-348 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1038/s41423-024-01125-1 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 21 |j 2024 |e 4 |b 10 |c 04 |h 332-348 | ||