A Mutagen Acts as a Potent Reducing Agent of Glycated Hemoglobin : a Combined Ultrafast Electron Transfer and Computational Studies
© 2024 Wiley-VCH GmbH..
Glycated hemoglobin (GHb) found in mammals undergoes irreversible damage when exposed to external redox agents, which is much more vulnerable than its normal counterpart hemoglobin (Hb). Besides the oxygen regulation throughout the body, Hb plays a vital role in balancing immunological health and the redox cycle. Photoinduced ultra-fast electron transfer phenomena actively participate in regulation of various kind of homeostasis involved in such biomacromolecules. In the present study we have shown that a well-known mutagen Ethidium Bromide (EtBr) reduces GHb in femtosecond time scale (efficiently) upon photoexcitation after efficient recognition in the biomolecule. We have performed similar experiment by colocalizing EtBr and Iron (Fe(III)) on the micellar surface as Hb mimic in order to study the excited state EtBr dynamics to rationalize the time scale obtained from EtBr in GHb and Hb. While other experimental techniques including Dynamic Light Scattering (DLS), Zeta potential, absorbance and emission spectroscopy have been employed for the confirmation of structural perturbation of GHb compared to Hb, a detailed computational studies involving molecular docking and density functional theory (DFT) have been employed for the explanation of the experimental observations.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
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| Enthalten in: |
Chembiochem : a European journal of chemical biology - 25(2024), 5 vom: 01. März, Seite e202300721 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Roy, Lopamudra [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 04.03.2024 Date Revised 26.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/cbic.202300721 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Glycated hemoglobin (GHb) found in mammals undergoes irreversible damage when exposed to external redox agents, which is much more vulnerable than its normal counterpart hemoglobin (Hb). Besides the oxygen regulation throughout the body, Hb plays a vital role in balancing immunological health and the redox cycle. Photoinduced ultra-fast electron transfer phenomena actively participate in regulation of various kind of homeostasis involved in such biomacromolecules. In the present study we have shown that a well-known mutagen Ethidium Bromide (EtBr) reduces GHb in femtosecond time scale (efficiently) upon photoexcitation after efficient recognition in the biomolecule. We have performed similar experiment by colocalizing EtBr and Iron (Fe(III)) on the micellar surface as Hb mimic in order to study the excited state EtBr dynamics to rationalize the time scale obtained from EtBr in GHb and Hb. While other experimental techniques including Dynamic Light Scattering (DLS), Zeta potential, absorbance and emission spectroscopy have been employed for the confirmation of structural perturbation of GHb compared to Hb, a detailed computational studies involving molecular docking and density functional theory (DFT) have been employed for the explanation of the experimental observations | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Ethidium bromide (EtBr) | |
| 650 | 4 | |a HbA1 and HbA1c | |
| 650 | 4 | |a biomacromolecular recognition | |
| 650 | 4 | |a electron transfer | |
| 650 | 4 | |a femtosecond resolved spectroscopy | |
| 650 | 7 | |a Glycated Hemoglobin |2 NLM | |
| 650 | 7 | |a Reducing Agents |2 NLM | |
| 650 | 7 | |a Mutagens |2 NLM | |
| 650 | 7 | |a Ferric Compounds |2 NLM | |
| 650 | 7 | |a Sodium Oxybate |2 NLM | |
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| 700 | 1 | |a Hasan, Md Nur |e verfasserin |4 aut | |
| 700 | 1 | |a Mondal, Susmita |e verfasserin |4 aut | |
| 700 | 1 | |a Banerjee, Amrita |e verfasserin |4 aut | |
| 700 | 1 | |a Das, Monojit |e verfasserin |4 aut | |
| 700 | 1 | |a Sen, Oyshi |e verfasserin |4 aut | |
| 700 | 1 | |a Bhattacharya, Kallol |e verfasserin |4 aut | |
| 700 | 1 | |a Chattopadhyay, Arpita |e verfasserin |4 aut | |
| 700 | 1 | |a Pal, Samir Kumar |e verfasserin |4 aut | |
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