Modulations of functional traits of Spinacia oleracea plants exposed to cadmium stress by using H2S as an antidote : a regulatory mechanism

© Prof. H.S. Srivastava Foundation for Science and Society 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law..

The present study is based on the application of H2S as an exogenous antidote in Spinacia oleracea (spinach) plants grown in Cd-contaminated (50 ppm) soil. The different doses of H2S in the form of NaHS (10, 50, 100, 200, and 500 μM) have been applied as a foliar spray to regulate the physiological attributes under Cd toxicity. Over to control, the plants grown in Cd alone showed a reduction in the fresh biomass by 48% with more production of oxidative biomarkers (H2O2, SOR, and MDA content) and antioxidative enzymes (SOD, POD, APX, and GR). Further, with the exogenous application of H2S, among all the doses the fresh biomass was found to be maximally increased at 100 μM dose by 76%, and the Cd content was reduced significantly by 25% in the shoot compared to plants grown in Cd treated soil alone. With the decrease in Cd content in the shoot, the production of H2O2, SOR, and MDA content was reduced by 52%, 40%, and 38% respectively, at 100 μM compared to the plants grown in Cd-treated soil. The activities of estimated antioxidative enzymes showed a reduction in their activities up to 100 μM. Whereas, Glutathione reductase (GR) and Phytochelatins (PCs) showed different trends with their higher values in plants treated with NaHS in the presence of Cd. At 100 μM the GR and PCs, respectively showed 48% and 37% increment over Cd-treated plants alone. At this dose, the relative expression of SOD, POD, APX, GR, and PCS5 (Phytochelatin synthetase enzyme) genes, and other functional activities (SEM and fluorescence kinetics) supported the best performance of plants at 100 μM. Therefore, among all the doses, 100 μM dose of H2S has significantly reduced the Cd toxicity by maintaining the growth and other functional traits of plants. The correlation analysis also supported the result by showing a relationship between H2S application and Cd uptake. So, with this strategy, the plants grown in metal-contaminated fields can be improved qualitatively as well as quantitatively. With further experimentation, the mode of application could be explored to increase its efficiency and to promote this strategy at a wider scale.

Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-023-01389-3.

Medienart:	E-Artikel

Erscheinungsjahr:	2023
Erschienen:	2023

Enthalten in:	Zur Gesamtaufnahme - volume:29
Enthalten in:	Physiology and molecular biology of plants : an international journal of functional plant biology - 29(2023), 12 vom: 19. Dez., Seite 2021-2033

Sprache:	Englisch

Beteiligte Personen:	Yadav, Pradeep Kumar [VerfasserIn] Kumar, Arun [VerfasserIn] Pandey, Prashasti [VerfasserIn] Kumar, Deepak [VerfasserIn] Singh, Anita [VerfasserIn]

Links:	Volltext

Themen:	Antioxidant Growth Journal Article Metals Signaling molecule Stress

Anmerkungen:	Date Revised 16.01.2024 published: Print-Electronic Citation Status PubMed-not-MEDLINE

doi:	10.1007/s12298-023-01389-3

funding:
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PPN (Katalog-ID):	NLM367128233