TKT-PARP1 axis induces radioresistance by promoting DNA double-strand break repair in hepatocellular carcinoma
© 2024. The Author(s)..
Hepatocellular carcinoma (HCC) stands as the fifth most prevalent malignant tumor on a global scale and presents as the second leading cause of cancer-related mortality. DNA damage-based radiotherapy (RT) plays a pivotal role in the treatment of HCC. Nevertheless, radioresistance remains a primary factor contributing to the failure of radiation therapy in HCC patients. In this study, we investigated the functional role of transketolase (TKT) in the repair of DNA double-strand breaks (DSBs) in HCC. Our research unveiled that TKT is involved in DSB repair, and its depletion significantly reduces both non-homologous end joining (NHEJ) and homologous recombination (HR)-mediated DSB repair. Mechanistically, TKT interacts with PARP1 in a DNA damage-dependent manner. Furthermore, TKT undergoes PARylation by PARP1, resulting in the inhibition of its enzymatic activity, and TKT can enhance the auto-PARylation of PARP1 in response to DSBs in HCC. The depletion of TKT effectively mitigates the radioresistance of HCC, both in vitro and in mouse xenograft models. Moreover, high TKT expression confers resistance of RT in clinical HCC patients, establishing TKT as a marker for assessing the response of HCC patients who received cancer RT. In summary, our findings reveal a novel mechanism by which TKT contributes to the radioresistance of HCC. Overall, we identify the TKT-PARP1 axis as a promising potential therapeutic target for improving RT outcomes in HCC.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:43 |
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| Enthalten in: |
Oncogene - 43(2024), 9 vom: 07. Feb., Seite 682-692 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Geng, Longpo [VerfasserIn] |
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| Links: |
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| Themen: |
9007-49-2 |
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| Anmerkungen: |
Date Completed 26.02.2024 Date Revised 27.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41388-023-02935-9 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM367072211 |
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| 245 | 1 | 0 | |a TKT-PARP1 axis induces radioresistance by promoting DNA double-strand break repair in hepatocellular carcinoma |
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| 520 | |a © 2024. The Author(s). | ||
| 520 | |a Hepatocellular carcinoma (HCC) stands as the fifth most prevalent malignant tumor on a global scale and presents as the second leading cause of cancer-related mortality. DNA damage-based radiotherapy (RT) plays a pivotal role in the treatment of HCC. Nevertheless, radioresistance remains a primary factor contributing to the failure of radiation therapy in HCC patients. In this study, we investigated the functional role of transketolase (TKT) in the repair of DNA double-strand breaks (DSBs) in HCC. Our research unveiled that TKT is involved in DSB repair, and its depletion significantly reduces both non-homologous end joining (NHEJ) and homologous recombination (HR)-mediated DSB repair. Mechanistically, TKT interacts with PARP1 in a DNA damage-dependent manner. Furthermore, TKT undergoes PARylation by PARP1, resulting in the inhibition of its enzymatic activity, and TKT can enhance the auto-PARylation of PARP1 in response to DSBs in HCC. The depletion of TKT effectively mitigates the radioresistance of HCC, both in vitro and in mouse xenograft models. Moreover, high TKT expression confers resistance of RT in clinical HCC patients, establishing TKT as a marker for assessing the response of HCC patients who received cancer RT. In summary, our findings reveal a novel mechanism by which TKT contributes to the radioresistance of HCC. Overall, we identify the TKT-PARP1 axis as a promising potential therapeutic target for improving RT outcomes in HCC | ||
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| 700 | 1 | |a Zhu, Mingming |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Dongjun |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Huihui |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Binghua |e verfasserin |4 aut | |
| 700 | 1 | |a Lao, Yuanxiang |e verfasserin |4 aut | |
| 700 | 1 | |a An, Hongda |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Yue |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yunzheng |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Anliang |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Tong, Zhuting |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Shanshan |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Dengqiu |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xu |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wenjun |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Beicheng |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Zhu |e verfasserin |4 aut | |
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