Red blood cells as oxygen carrier during normothermic machine perfusion of kidney grafts : Friend or foe?
Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved..
Renal ex vivo normothermic machine perfusion (NMP) is under development as an assessment tool for high-risk kidney grafts and as a means of achieving more physiologically accurate organ preservation. On-going hemolysis has been reported during NMP, as this technique relies on red blood cells for oxygen delivery. In this study, we confirm the occurrence of progressive hemolysis during 6-hour kidney NMP. NMP-associated erythrostasis in the glomeruli and in peri-glomerular vascular networks points to an interaction between the red blood cells and the graft. Continuous hemolysis resulted in prooxidative changes in the perfusate, which could be quenched by addition of fresh frozen plasma. In a cell-based system, this hemolysis induced redox stress and exhibited toxic effects at high concentrations. These findings highlight the need for a more refined oxygen carrier in the context of renal NMP.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons - (2024) vom: 11. Jan. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Arykbaeva, Asel S [VerfasserIn] |
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| Links: |
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| Themen: |
Journal Article |
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| Anmerkungen: |
Date Revised 24.01.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1016/j.ajt.2024.01.002 |
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| PPN (Katalog-ID): |
NLM367065312 |
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| 520 | |a Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Renal ex vivo normothermic machine perfusion (NMP) is under development as an assessment tool for high-risk kidney grafts and as a means of achieving more physiologically accurate organ preservation. On-going hemolysis has been reported during NMP, as this technique relies on red blood cells for oxygen delivery. In this study, we confirm the occurrence of progressive hemolysis during 6-hour kidney NMP. NMP-associated erythrostasis in the glomeruli and in peri-glomerular vascular networks points to an interaction between the red blood cells and the graft. Continuous hemolysis resulted in prooxidative changes in the perfusate, which could be quenched by addition of fresh frozen plasma. In a cell-based system, this hemolysis induced redox stress and exhibited toxic effects at high concentrations. These findings highlight the need for a more refined oxygen carrier in the context of renal NMP | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a kidney transplantation/nephrology | |
| 650 | 4 | |a normothermic machine perfusion | |
| 650 | 4 | |a organ perfusion and preservation | |
| 650 | 4 | |a oxygen carrier | |
| 650 | 4 | |a red blood cells | |
| 700 | 1 | |a Lerink, Lente J S |e verfasserin |4 aut | |
| 700 | 1 | |a Vos, Jaël |e verfasserin |4 aut | |
| 700 | 1 | |a Engelse, Marten A |e verfasserin |4 aut | |
| 700 | 1 | |a van Kooten, Cees |e verfasserin |4 aut | |
| 700 | 1 | |a de Korte, Dirk |e verfasserin |4 aut | |
| 700 | 1 | |a Lagerberg, Johan W M |e verfasserin |4 aut | |
| 700 | 1 | |a Klei, Thomas R L |e verfasserin |4 aut | |
| 700 | 1 | |a Mulder, Aat A |e verfasserin |4 aut | |
| 700 | 1 | |a Minnee, Robert C |e verfasserin |4 aut | |
| 700 | 1 | |a Ploeg, Rutger J |e verfasserin |4 aut | |
| 700 | 1 | |a Moers, Cyril |e verfasserin |4 aut | |
| 700 | 1 | |a Pol, Robert A |e verfasserin |4 aut | |
| 700 | 1 | |a Alwayn, Ian P J |e verfasserin |4 aut | |
| 700 | 1 | |a de Vries, Dorottya K |e verfasserin |4 aut | |
| 700 | 1 | |a Lindeman, Jan H N |e verfasserin |4 aut | |
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