mRNA COVID-19 vaccine elicits potent adaptive immune response without the acute inflammation of SARS-CoV-2 infection
© 2023 The Authors..
SARS-CoV-2 infection and vaccination elicit potent immune responses. Our study presents a comprehensive multimodal single-cell analysis of blood from COVID-19 patients and healthy volunteers receiving the SARS-CoV-2 vaccine and booster. We profiled immune responses via transcriptional analysis and lymphocyte repertoire reconstruction. COVID-19 patients displayed an enhanced interferon signature and cytotoxic gene upregulation, absent in vaccine recipients. B and T cell repertoire analysis revealed clonal expansion among effector cells in COVID-19 patients and memory cells in vaccine recipients. Furthermore, while clonal αβ T cell responses were observed in both COVID-19 patients and vaccine recipients, expansion of clonal γδ T cells was found only in infected individuals. Our dataset enables side-by-side comparison of immune responses to infection versus vaccination, including clonal B and T cell responses. Our comparative analysis shows that vaccination induces a robust, durable clonal B and T cell responses, without the severe inflammation associated with infection.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:26 |
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| Enthalten in: |
iScience - 26(2023), 12 vom: 15. Dez., Seite 108572 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ivanova, Ellie N [VerfasserIn] |
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Date Revised 10.02.2024 published: Electronic-eCollection UpdateOf: medRxiv. 2023 Aug 23;:. - PMID 33907755 Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.isci.2023.108572 |
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| funding: |
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| PPN (Katalog-ID): |
NLM367043432 |
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| 520 | |a © 2023 The Authors. | ||
| 520 | |a SARS-CoV-2 infection and vaccination elicit potent immune responses. Our study presents a comprehensive multimodal single-cell analysis of blood from COVID-19 patients and healthy volunteers receiving the SARS-CoV-2 vaccine and booster. We profiled immune responses via transcriptional analysis and lymphocyte repertoire reconstruction. COVID-19 patients displayed an enhanced interferon signature and cytotoxic gene upregulation, absent in vaccine recipients. B and T cell repertoire analysis revealed clonal expansion among effector cells in COVID-19 patients and memory cells in vaccine recipients. Furthermore, while clonal αβ T cell responses were observed in both COVID-19 patients and vaccine recipients, expansion of clonal γδ T cells was found only in infected individuals. Our dataset enables side-by-side comparison of immune responses to infection versus vaccination, including clonal B and T cell responses. Our comparative analysis shows that vaccination induces a robust, durable clonal B and T cell responses, without the severe inflammation associated with infection | ||
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| 700 | 1 | |a Devlin, Joseph C |e verfasserin |4 aut | |
| 700 | 1 | |a Buus, Terkild B |e verfasserin |4 aut | |
| 700 | 1 | |a Gray-Gaillard, Sophie |e verfasserin |4 aut | |
| 700 | 1 | |a Koide, Akiko |e verfasserin |4 aut | |
| 700 | 1 | |a Cornelius, Amber |e verfasserin |4 aut | |
| 700 | 1 | |a Samanovic, Marie I |e verfasserin |4 aut | |
| 700 | 1 | |a Herrera, Alberto |e verfasserin |4 aut | |
| 700 | 1 | |a Mimitou, Eleni P |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Chenzhen |e verfasserin |4 aut | |
| 700 | 1 | |a Karmacharya, Trishala |e verfasserin |4 aut | |
| 700 | 1 | |a Desvignes, Ludovic |e verfasserin |4 aut | |
| 700 | 1 | |a Ødum, Niels |e verfasserin |4 aut | |
| 700 | 1 | |a Smibert, Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Ulrich, Robert J |e verfasserin |4 aut | |
| 700 | 1 | |a Mulligan, Mark J |e verfasserin |4 aut | |
| 700 | 1 | |a Koide, Shohei |e verfasserin |4 aut | |
| 700 | 1 | |a Ruggles, Kelly V |e verfasserin |4 aut | |
| 700 | 1 | |a Herati, Ramin S |e verfasserin |4 aut | |
| 700 | 1 | |a Koralov, Sergei B |e verfasserin |4 aut | |
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