Senescence on Dental Pulp Cells : Effects on Morphology, Migration, Proliferation, and Immune Response
Copyright © 2024 American Association of Endodontists. Published by Elsevier Inc. All rights reserved..
INTRODUCTION: Evidence indicates that senescence can affect essential dental pulp functions, such as defense capacity and repair, consequently affecting the successes of conservative endodontic treatments. This study aims to evaluate the effects of senescence on the morphology, migration, proliferation, and immune response of human dental pulp cells.
METHODS: Cells were treated with doxorubicin to induce senescence, confirmed by β-galactosidase staining. Morphological changes, cellular proliferation, and migration were evaluated by scanning electron microscopy, trypan blue cells, and the scratch method, respectively. Modifications in the immune response were evaluated by measuring the genes for pro-inflammatory cytokines tumor necrosis factor alpha and interleukin (IL)-6 and anti-inflammatory cytokines transforming growth factor beta 1 and IL-10 using the real time polymerase chain reaction assay.
RESULTS: An increase in cell size and a decrease in the number of extensions were observed in senescent cells. A reduction in the proliferative and migratory capacity was also found in senescent cells. In addition, there was an increase in the gene expression of inflammatory cytokines tumor necrosis factor alpha and IL-6 and a decrease in the gene expression of IL-10 and transforming growth factor beta-1, suggesting an exacerbated inflammatory situation associated with immunosuppression.
CONCLUSIONS: Cellular senescence is possibly a condition that affects prognoses of conservative endodontic treatments, as it affects primordial cellular functions related to this treatment.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:50 |
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| Enthalten in: |
Journal of endodontics - 50(2024), 3 vom: 15. Feb., Seite 362-369 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
de Farias, Jade Ormondes [VerfasserIn] |
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| Links: |
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| Themen: |
130068-27-8 |
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| Anmerkungen: |
Date Completed 27.02.2024 Date Revised 27.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.joen.2023.12.009 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2024 American Association of Endodontists. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a INTRODUCTION: Evidence indicates that senescence can affect essential dental pulp functions, such as defense capacity and repair, consequently affecting the successes of conservative endodontic treatments. This study aims to evaluate the effects of senescence on the morphology, migration, proliferation, and immune response of human dental pulp cells | ||
| 520 | |a METHODS: Cells were treated with doxorubicin to induce senescence, confirmed by β-galactosidase staining. Morphological changes, cellular proliferation, and migration were evaluated by scanning electron microscopy, trypan blue cells, and the scratch method, respectively. Modifications in the immune response were evaluated by measuring the genes for pro-inflammatory cytokines tumor necrosis factor alpha and interleukin (IL)-6 and anti-inflammatory cytokines transforming growth factor beta 1 and IL-10 using the real time polymerase chain reaction assay | ||
| 520 | |a RESULTS: An increase in cell size and a decrease in the number of extensions were observed in senescent cells. A reduction in the proliferative and migratory capacity was also found in senescent cells. In addition, there was an increase in the gene expression of inflammatory cytokines tumor necrosis factor alpha and IL-6 and a decrease in the gene expression of IL-10 and transforming growth factor beta-1, suggesting an exacerbated inflammatory situation associated with immunosuppression | ||
| 520 | |a CONCLUSIONS: Cellular senescence is possibly a condition that affects prognoses of conservative endodontic treatments, as it affects primordial cellular functions related to this treatment | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a da Costa Sousa, Maurício Gonçalves |e verfasserin |4 aut | |
| 700 | 1 | |a Martins, Danilo César Mota |e verfasserin |4 aut | |
| 700 | 1 | |a de Oliveira, Mayara Alves |e verfasserin |4 aut | |
| 700 | 1 | |a Takahashi, Isadora |e verfasserin |4 aut | |
| 700 | 1 | |a de Sousa, Larissa Barbosa |e verfasserin |4 aut | |
| 700 | 1 | |a da Silva, Ingrid Gracielle Martins |e verfasserin |4 aut | |
| 700 | 1 | |a Corrêa, José Raimundo |e verfasserin |4 aut | |
| 700 | 1 | |a Silva Carvalho, Amandda Évelin |e verfasserin |4 aut | |
| 700 | 1 | |a Saldanha-Araújo, Felipe |e verfasserin |4 aut | |
| 700 | 1 | |a Rezende, Taia Maria Berto |e verfasserin |4 aut | |
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