Distribution and morphology of calcitonin gene-related peptide (CGRP) innervation in flat mounts of whole rat atria and ventricles
Copyright © 2023 Elsevier B.V. All rights reserved..
Calcitonin gene-related peptide (CGRP) is widely used as a marker for nociceptive afferent axons. However, the distribution of CGRP-IR axons has not been fully determined in the whole rat heart. Immunohistochemically labeled flat-mounts of the right and left atria and ventricles, and the interventricular septum (IVS) in rats for CGRP were assessed with a Zeiss imager to generate complete montages of the entire atria, ventricles, and septum, and a confocal microscope was used to acquire detailed images of selected regions. We found that 1) CGRP-IR axons extensively innervated all regions of the atrial walls including the sinoatrial node region, auricles, atrioventricular node region, superior/inferior vena cava, left pre-caval vein, and pulmonary veins. 2) CGRP-IR axons formed varicose terminals around individual neurons in some cardiac ganglia but passed through other ganglia without making appositions with cardiac neurons. 3) Varicose CGRP-IR axons innervated the walls of blood vessels. 4) CGRP-IR axons extensively innervated the right/left ventricular walls and IVS. Our data shows the rather ubiquitous distribution of CGRP-IR axons in the whole rat heart at single-cell/axon/varicosity resolution for the first time. This study lays the foundation for future studies to quantify the differences in CGRP-IR axon innervation between sexes, disease models, and species.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:251 |
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| Enthalten in: |
Autonomic neuroscience : basic & clinical - 251(2024) vom: 01. Jan., Seite 103127 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chen, Jin [VerfasserIn] |
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| Links: |
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| Themen: |
Atria |
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| Anmerkungen: |
Date Completed 29.01.2024 Date Revised 29.01.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.autneu.2023.103127 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM367019337 |
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| 520 | |a Calcitonin gene-related peptide (CGRP) is widely used as a marker for nociceptive afferent axons. However, the distribution of CGRP-IR axons has not been fully determined in the whole rat heart. Immunohistochemically labeled flat-mounts of the right and left atria and ventricles, and the interventricular septum (IVS) in rats for CGRP were assessed with a Zeiss imager to generate complete montages of the entire atria, ventricles, and septum, and a confocal microscope was used to acquire detailed images of selected regions. We found that 1) CGRP-IR axons extensively innervated all regions of the atrial walls including the sinoatrial node region, auricles, atrioventricular node region, superior/inferior vena cava, left pre-caval vein, and pulmonary veins. 2) CGRP-IR axons formed varicose terminals around individual neurons in some cardiac ganglia but passed through other ganglia without making appositions with cardiac neurons. 3) Varicose CGRP-IR axons innervated the walls of blood vessels. 4) CGRP-IR axons extensively innervated the right/left ventricular walls and IVS. Our data shows the rather ubiquitous distribution of CGRP-IR axons in the whole rat heart at single-cell/axon/varicosity resolution for the first time. This study lays the foundation for future studies to quantify the differences in CGRP-IR axon innervation between sexes, disease models, and species | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Atria | |
| 650 | 4 | |a Calcitonin gene-related peptide | |
| 650 | 4 | |a Heart | |
| 650 | 4 | |a Intrinsic cardiac ganglia | |
| 650 | 4 | |a Vasculature | |
| 650 | 4 | |a Ventricles | |
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| 700 | 1 | |a Bendowski, Kohlton T |e verfasserin |4 aut | |
| 700 | 1 | |a Bizanti, Ariege |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yuanyuan |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Jichao |e verfasserin |4 aut | |
| 700 | 1 | |a Hoover, Donald B |e verfasserin |4 aut | |
| 700 | 1 | |a Gozal, David |e verfasserin |4 aut | |
| 700 | 1 | |a Shivkumar, Kalyanam |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Zixi Jack |e verfasserin |4 aut | |
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