The Assessment of Serum Fibronectin Levels as a Potential Biomarker for the Severity of Drug-Sensitive Pulmonary Tuberculosis : A Pilot Study
BACKGROUND: Tuberculosis (TB) is a global health burden caused by Mycobacterium tuberculosis (Mtb) infection. Fibronectin (Fn) facilitates Mtb attachment to host cells. We studied the Fn levels in smear-positive TB patients to assess its correlation with disease severity based on sputum smears and chest X-rays.
METHODS: Newly detected consecutive sputum AFB-positive pulmonary TB patients (n = 78) and healthy control subjects (n = 11) were included. The mycobacterial load in the sputum smear was assessed by IUATLD classification, ranging from 0 to 3. The severity of pulmonary involvement was assessed radiologically in terms of both the number of zones involved (0-6) and as localized (up to 2 zones), moderate (3-4 zones), or extensive (5-6 zones). The serum human fibronectin levels were measured by using a commercially available enzyme-linked immunosorbent assay (ELISA) kit (Catalogue No: CK-bio-11486, Shanghai Coon Koon Biotech Co., Ltd., Shanghai, China).
RESULTS: The PTB patients showed lower Fn levels (102.4 ± 26.7) compared with the controls (108.8 ± 6.8), but they were not statistically significant. Higher AFB smear grades had lower Fn levels. The chest X-ray zones involved were inversely correlated with Fn levels. The Fn levels, adjusted for age and gender, decreased with increased mycobacterial load and the number of chest radiograph zones affected. A Fn level <109.39 g/mL predicted greater TB severity (sensitivity of 67.57% and specificity of 90.38%), while a level <99.32 pg/mL predicted severity based on the chest radiology (sensitivity of 84.21% and specificity of 100%).
CONCLUSIONS: The Fn levels are lower in tuberculosis patients and are negatively correlated with severity based on sputum mycobacterial load and chest radiographs. The Fn levels may serve as a potential biomarker for assessing TB severity, which could have implications for early diagnosis and treatment monitoring.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2023 |
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Erschienen: |
2023 |
Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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Enthalten in: |
Diagnostics (Basel, Switzerland) - 14(2023), 1 vom: 25. Dez. |
Sprache: |
Englisch |
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Beteiligte Personen: |
Kulkarni, Shreedhar [VerfasserIn] |
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Links: |
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Themen: |
Disease severity |
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Anmerkungen: |
Date Revised 01.02.2024 published: Electronic Citation Status PubMed-not-MEDLINE |
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doi: |
10.3390/diagnostics14010050 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM366919458 |
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100 | 1 | |a Kulkarni, Shreedhar |e verfasserin |4 aut | |
245 | 1 | 4 | |a The Assessment of Serum Fibronectin Levels as a Potential Biomarker for the Severity of Drug-Sensitive Pulmonary Tuberculosis |b A Pilot Study |
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520 | |a BACKGROUND: Tuberculosis (TB) is a global health burden caused by Mycobacterium tuberculosis (Mtb) infection. Fibronectin (Fn) facilitates Mtb attachment to host cells. We studied the Fn levels in smear-positive TB patients to assess its correlation with disease severity based on sputum smears and chest X-rays | ||
520 | |a METHODS: Newly detected consecutive sputum AFB-positive pulmonary TB patients (n = 78) and healthy control subjects (n = 11) were included. The mycobacterial load in the sputum smear was assessed by IUATLD classification, ranging from 0 to 3. The severity of pulmonary involvement was assessed radiologically in terms of both the number of zones involved (0-6) and as localized (up to 2 zones), moderate (3-4 zones), or extensive (5-6 zones). The serum human fibronectin levels were measured by using a commercially available enzyme-linked immunosorbent assay (ELISA) kit (Catalogue No: CK-bio-11486, Shanghai Coon Koon Biotech Co., Ltd., Shanghai, China) | ||
520 | |a RESULTS: The PTB patients showed lower Fn levels (102.4 ± 26.7) compared with the controls (108.8 ± 6.8), but they were not statistically significant. Higher AFB smear grades had lower Fn levels. The chest X-ray zones involved were inversely correlated with Fn levels. The Fn levels, adjusted for age and gender, decreased with increased mycobacterial load and the number of chest radiograph zones affected. A Fn level <109.39 g/mL predicted greater TB severity (sensitivity of 67.57% and specificity of 90.38%), while a level <99.32 pg/mL predicted severity based on the chest radiology (sensitivity of 84.21% and specificity of 100%) | ||
520 | |a CONCLUSIONS: The Fn levels are lower in tuberculosis patients and are negatively correlated with severity based on sputum mycobacterial load and chest radiographs. The Fn levels may serve as a potential biomarker for assessing TB severity, which could have implications for early diagnosis and treatment monitoring | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Mycobacterium tuberculosis | |
650 | 4 | |a disease severity | |
650 | 4 | |a fibronectin | |
650 | 4 | |a mycobacterial load | |
650 | 4 | |a sputum positive PTB | |
700 | 1 | |a Arunachala, Sumalatha |e verfasserin |4 aut | |
700 | 1 | |a Chaya, Sindaghatta Krishnarao |e verfasserin |4 aut | |
700 | 1 | |a ShankaraSetty, Rekha Vaddarahalli |e verfasserin |4 aut | |
700 | 1 | |a Karnik, Medha |e verfasserin |4 aut | |
700 | 1 | |a Bansal, Nidhi |e verfasserin |4 aut | |
700 | 1 | |a Ravindran, Sukanya |e verfasserin |4 aut | |
700 | 1 | |a Lokesh, Komarla Sundararaja |e verfasserin |4 aut | |
700 | 1 | |a Mohan, Mikash |e verfasserin |4 aut | |
700 | 1 | |a Kaleem Ullah, Mohammed |e verfasserin |4 aut | |
700 | 1 | |a Siddaiah, Jayaraj Biligere |e verfasserin |4 aut | |
700 | 1 | |a Mahesh, Padukudru Anand |e verfasserin |4 aut | |
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