IiAGL6 participates in the regulation of stamen development and pollen formation in Isatis indigotica
Copyright © 2024 Elsevier B.V. All rights reserved..
The AGL6 (AGMOUSE LIKE 6) gene is a member of the SEP subfamily and functions as an E-class floral homeotic gene in the development of floral organs. In this study, we cloned IiAGL6, the orthologous gene of AGL6 in Isatis indigotica. The constitutive expression of IiAGL6 in Arabidopsis thaliana resulted in a late-flowering phenotype and the development of curly leaves during the vegetative growth period. Abnormal changes in floral organ development were observed during the reproductive stage. In woad plants, suppression of IiAGL6 using TRV-VIGS (tobacco rattle virus-mediated virus-induced gene silencing) decreased the number of stamens and led to the formation of aberrant anthers. Similar changes in stamen development were also observed in miRNA-AGL6 transgenic Arabidopsis plants. Yeast two-hybrid and BiFC tests showed that IiAGL6 can interact with other MADS-box proteins in woad; thus, playing a key role in defining the identities of floral organs, particularly during stamen formation. These findings might provide novel insights and help investigate the biological roles of MADS transcription factors in I. indigotica.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:340 |
|---|---|
| Enthalten in: |
Plant science : an international journal of experimental plant biology - 340(2024) vom: 01. Feb., Seite 111974 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Yang, Liu [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
AGMOUSE LIKE 6 |
|---|
| Anmerkungen: |
Date Completed 05.02.2024 Date Revised 05.02.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.plantsci.2024.111974 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM366899759 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM366899759 | ||
| 003 | DE-627 | ||
| 005 | 20240205232029.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240114s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.plantsci.2024.111974 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1281.xml |
| 035 | |a (DE-627)NLM366899759 | ||
| 035 | |a (NLM)38199385 | ||
| 035 | |a (PII)S0168-9452(24)00001-3 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Yang, Liu |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a IiAGL6 participates in the regulation of stamen development and pollen formation in Isatis indigotica |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 05.02.2024 | ||
| 500 | |a Date Revised 05.02.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024 Elsevier B.V. All rights reserved. | ||
| 520 | |a The AGL6 (AGMOUSE LIKE 6) gene is a member of the SEP subfamily and functions as an E-class floral homeotic gene in the development of floral organs. In this study, we cloned IiAGL6, the orthologous gene of AGL6 in Isatis indigotica. The constitutive expression of IiAGL6 in Arabidopsis thaliana resulted in a late-flowering phenotype and the development of curly leaves during the vegetative growth period. Abnormal changes in floral organ development were observed during the reproductive stage. In woad plants, suppression of IiAGL6 using TRV-VIGS (tobacco rattle virus-mediated virus-induced gene silencing) decreased the number of stamens and led to the formation of aberrant anthers. Similar changes in stamen development were also observed in miRNA-AGL6 transgenic Arabidopsis plants. Yeast two-hybrid and BiFC tests showed that IiAGL6 can interact with other MADS-box proteins in woad; thus, playing a key role in defining the identities of floral organs, particularly during stamen formation. These findings might provide novel insights and help investigate the biological roles of MADS transcription factors in I. indigotica | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a AGMOUSE LIKE 6 | |
| 650 | 4 | |a Arabidopsis thaliana | |
| 650 | 4 | |a Development of stamen | |
| 650 | 4 | |a Isatis indigotica fortune | |
| 650 | 4 | |a Virus-induced gene silencing | |
| 650 | 7 | |a Plant Proteins |2 NLM | |
| 650 | 7 | |a MADS Domain Proteins |2 NLM | |
| 700 | 1 | |a Qiao, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a He, Hao-En |e verfasserin |4 aut | |
| 700 | 1 | |a Yi, Wei-Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Ya-Nan |e verfasserin |4 aut | |
| 700 | 1 | |a Tan, Xiao-Min |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Hou, Xiao-Fang |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Ye-Ye |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Hong-Li |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Xuan |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Yan-Qin |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Zi-Qin |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Plant science : an international journal of experimental plant biology |d 1985 |g 340(2024) vom: 01. Feb., Seite 111974 |w (DE-627)NLM098174193 |x 1873-2259 |7 nnns |
| 773 | 1 | 8 | |g volume:340 |g year:2024 |g day:01 |g month:02 |g pages:111974 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.plantsci.2024.111974 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 340 |j 2024 |b 01 |c 02 |h 111974 | ||